Rapid and simple isolation of DNA from agarose gels.
作者信息
Grey M, Brendel M
机构信息
Institut für Mikrobiologie, J. W. Goethe-Universität, Frankfurt/Main, Federal Republic of Germany.
出版信息
Curr Genet. 1992 Jul;22(1):83-4. doi: 10.1007/BF00351746.
PMID:1611671
Abstract
We present a simple method for the isolation of DNA from agarose gels that is economic, fast, and independent of electrical equipment. DNA fragments of up to 6 kb can be easily extracted within 5 min using a disposable plastic syringe and filter paper. Total extraction of DNA fragments between 10 and 20 kb in size is achieved by concentrating the DNA flushed from the gel in a DNA-binding column.
摘要
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