Tanaka Daisuke, Isobe Junko, Kimata Keiko, Shima Tomoko, Watahiki Masanori
Department of Bacteriology, Toyama Institute of Health.
Kansenshogaku Zasshi. 2005 Jul;79(7):443-50. doi: 10.11150/kansenshogakuzasshi1970.79.443.
Group A Streptococci (GAS) from patients with pharyngitis and skin infections were examined for T serotypes, emm types, and streptococcal pyrogenic exotoxin gene types. The results were summarized as follows: 1) T and emm types were determined in 130 GAS isolates obtained between 2000 and 2004. Among 85 throat isolates, predominant T/emm types were T12/emm12 (25%), T4/emm4 (19%), and T1/emm1 (14%). Among 45 skin isolates, predominant T/emm types were T28/emm28 (13%), TB 3264/emm89 (13%), Tnontypeable/emm58 (13%), T1/emm1 (11%), and T12/emm12 (11%). Predominant T/emm types of skin isolates in 2000-2004 slightly differed from those during 1990s in our previous report. 2) The presence of streptococcal pyrogenic exotoxin genes in 292 GAS isolates obtained between 1990 and 2004 was examined. Significantly lower proportion of skin isolates, compared with throat isolates, was found to harbor the speA gene (12 versus 26%, respectively; p<0.01), or the speC gene (40 versus 65%, respectively; P<0.01). All but one of tested isolates carried the speB gene. The speB-negative isolate was identified as S. dysgalactiae subsp. equisimilis with the group A antigen. 3) Types of the speA alleles were determined in 59 speA-positive GAS isolates. Among 44 throat isolates, 37 (84%) were speA lineage I (speA1-speA2-speA3-speA6), and 7 (16%) were lineage II (speA4-speA5). Among 15 skin isolates, 11 (73%) were lineage I and 4 (27%) were lineage II. The pairwise associations were observed between emm type and speA allele: emm1 and speA2, emm3 and speA3, emm6 and speA4, emm11 and speA2, emm18 and speA1.
对咽炎和皮肤感染患者的A组链球菌（GAS）进行了T血清型、emm型和链球菌致热外毒素基因分型检测。结果总结如下：1）对2000年至2004年间获得的130株GAS分离株进行了T型和emm型检测。在85株咽喉分离株中，主要的T/emm型为T12/emm12（25%）、T4/emm4（19%）和T1/emm1（14%）。在45株皮肤分离株中，主要的T/emm型为T28/emm28（13%）、TB 3264/emm89（13%）、不可分型T/emm58（13%）、T1/emm1（11%）和T12/emm12（11%）。2000 - 2004年皮肤分离株的主要T/emm型与我们之前报告的20世纪90年代略有不同。2）检测了1990年至2004年间获得的292株GAS分离株中链球菌致热外毒素基因的存在情况。发现皮肤分离株中携带speA基因（分别为12%和26%；p<0.01）或speC基因（分别为40%和65%；P<0.01）的比例明显低于咽喉分离株。除一株检测分离株外，其余均携带speB基因。该speB阴性分离株被鉴定为具有A组抗原的马链球菌兽疫亚种。3）对59株speA阳性GAS分离株进行了speA等位基因分型。在44株咽喉分离株中，37株（84%）为speA谱系I（speA1 - speA2 - speA3 - speA6），7株（16%）为谱系II（speA4 - speA5）。在15株皮肤分离株中，11株（73%）为谱系I，4株（27%）为谱系II。观察到emm型与speA等位基因之间存在成对关联：emm1与speA2、emm3与speA3、emm6与speA4、emm11与speA2、emm18与speA1。
