Kadoya K, Sasaki T, Kostka G, Timpl R, Matsuzaki K, Kumagai N, Sakai L Y, Nishiyama T, Amano S
Skin Biology Research Laboratories, Shiseido Life Science Research Centre, 2-12-1 Fukuura, Kanazawa-ku, Yokohama-shi 236-8643, Japan.
Br J Dermatol. 2005 Sep;153(3):607-12. doi: 10.1111/j.1365-2133.2005.06716.x.
Fibulin-5 was recently found as a secreted extracellular matrix protein that functions as a scaffold for elastic fibres. However, the distribution of fibulin-5 in human skin and its changes during the ageing process are not known.
To explore the involvement of fibulin-5 in skin ageing, the age-dependent changes in fibulin-5 localization in human skin were examined compared with those of other elastic fibre components including elastin, fibrillin-1 and fibulin-2. Methods The distribution of elastin, fibrillin-1, fibrillin-2, fibulin-2 and fibulin-5 was investigated by means of immunohistochemistry using their specific antibodies. Skin samples were recovered from 12 healthy subjects undergoing plastic surgery. Ultraviolet (UV) B-irradiated or control nonirradiated buttock skin samples were obtained from two healthy volunteers at 2 days after the irradiation at 2 minimal erythemal doses.
In the reticular dermis of young sun-protected skin from the upper arm, fibulin-5 colocalized with the other elastic fibre components, while in the papillary dermis fibulin-5 showed candelabra-like structures perpendicular to the epidermis with an unstained area just beneath the epidermis, which was similar to that of elastin but not fibrillin-1. Fibulin-5 in the reticular dermis decreased and disappeared with age even in sun-protected skin from the thigh, abdomen and upper arm. In sun-exposed skin, fibulin-5 was extremely reduced in the dermis of cheek skin even from a 20-year-old man. UVB irradiation reduced fibulin-5, fibulin-2 and elastin markedly, moderately and weakly, respectively, compared with levels in control nontreated skin. Interestingly, the deposition of fibulin-5 was increased in solar elastosis, like that of other elastic fibre components.
These results suggest that fibulin-5 is a good marker of skin ageing and that the earlier loss of fibulin-5 may involve age-dependent changes in other elastic fibre components.
纤维连接蛋白-5最近被发现是一种分泌型细胞外基质蛋白，作为弹性纤维的支架发挥作用。然而，纤维连接蛋白-5在人体皮肤中的分布及其在衰老过程中的变化尚不清楚。
为了探究纤维连接蛋白-5在皮肤衰老中的作用，将人体皮肤中纤维连接蛋白-5定位的年龄依赖性变化与其他弹性纤维成分（包括弹性蛋白、原纤蛋白-1和纤维连接蛋白-2）的变化进行比较。方法使用特异性抗体通过免疫组织化学研究弹性蛋白、原纤蛋白-1、原纤蛋白-2、纤维连接蛋白-2和纤维连接蛋白-5的分布。从12名接受整形手术的健康受试者身上采集皮肤样本。在两名健康志愿者接受2个最小红斑剂量的紫外线（UV）B照射2天后，从其臀部获取紫外线B照射或对照未照射的皮肤样本。
在来自上臂的年轻防晒皮肤的网状真皮中，纤维连接蛋白-5与其他弹性纤维成分共定位，而在乳头真皮中，纤维连接蛋白-5呈现出垂直于表皮的烛台样结构，表皮正下方有一个未染色区域，这与弹性蛋白相似，但与原纤蛋白-1不同。即使在来自大腿、腹部和上臂的防晒皮肤中，网状真皮中的纤维连接蛋白-5也会随着年龄增长而减少并消失。在暴露于阳光的皮肤中，即使是一名20岁男性的脸颊皮肤真皮中，纤维连接蛋白-5也极度减少。与未处理的对照皮肤相比，紫外线B照射分别使纤维连接蛋白-5、纤维连接蛋白-2和弹性蛋白明显、中度和轻度减少。有趣的是，与其他弹性纤维成分一样，在日光性弹力纤维变性中纤维连接蛋白-5的沉积增加。
这些结果表明，纤维连接蛋白-5是皮肤衰老的一个良好标志物，纤维连接蛋白-5的早期丧失可能与其他弹性纤维成分的年龄依赖性变化有关。
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