Ignatova Zoya
Biotechnologie II, Technische Universität Hamburg-Harburg, Denickestr. 15, 21073 Hamburg, Germany.
Microb Cell Fact. 2005 Aug 24;4:23. doi: 10.1186/1475-2859-4-23.
Reduced protein stability in vivo is a prerequisite to aggregation. While this is merely a nuisance factor in recombinant protein production, it holds a serious impact for man. This review focuses on specific approaches to selectively determine the solubility and/or stability of a target protein within the complex cellular environment using different detection techniques. Noninvasive techniques mapping folding/misfolding events on a fast time scale can be used to unravel the complexity and dynamics of the protein aggregation process and factors altering protein solubility in vivo. The development of approaches to screen for folding and solubility in vivo should facilitate the identification of potential components that improve protein solubility and/or modulate misfolding and aggregation and may provide a therapeutic benefit.
体内蛋白质稳定性降低是聚集的前提条件。虽然这在重组蛋白生产中仅仅是一个干扰因素，但对人类却有着严重影响。本综述聚焦于使用不同检测技术在复杂细胞环境中选择性测定目标蛋白溶解度和/或稳定性的特定方法。能够在快速时间尺度上绘制折叠/错误折叠事件的非侵入性技术，可用于揭示蛋白质聚集过程的复杂性和动态性，以及改变体内蛋白质溶解度的因素。开发在体内筛选折叠和溶解度的方法，应有助于识别改善蛋白质溶解度和/或调节错误折叠及聚集的潜在成分，并可能带来治疗益处。
