b型流感嗜血杆菌在连续培养中的生长：稀释率对外膜蛋白和脂多糖表达的影响。

Growth of Haemophilus influenzae type b in continuous culture: effect of dilution rate on outer-membrane protein and lipopolysaccharide expression.

作者信息

Langford P R, Moxon E R

机构信息

Department of Paediatrics, Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK.

出版信息

FEMS Microbiol Lett. 1992 May 15;72(1):43-7. doi: 10.1016/0378-1097(92)90487-9.

DOI:10.1016/0378-1097(92)90487-9

PMID:1612416

Abstract

Haemophilus influenzae type b (Hib) was grown in continuous culture under cystine-limitation between dilution rates (D) of 0.065-0.28 h-1. A similar outer-membrane protein profile, as adjudged by SDS-PAGE, was found at all dilution rates. However, a shift to a lipopolysaccharide structure with a greater electrophoretic mobility on SDS-PAGE with accompanying changes in monoclonal antibody reactivity was observed at D greater than or equal to 0.15 h-1. Growth rate per se can affect the expression of outer-membrane components of Hib.

摘要

b型流感嗜血杆菌（Hib）在胱氨酸限制条件下，于稀释率（D）为0.065 - 0.28 h⁻¹之间进行连续培养。通过SDS - PAGE判断，在所有稀释率下均发现了相似的外膜蛋白谱。然而，在D大于或等于0.15 h⁻¹时，观察到在SDS - PAGE上具有更高电泳迁移率的脂多糖结构发生了转变，同时单克隆抗体反应性也发生了变化。生长速率本身会影响Hib外膜成分的表达。