ADP核糖基化在灰色链霉菌孢子形成生理调节中的可能作用。

The possible role of ADP ribosylation in physiological regulation of sporulation in Streptomyces griseus.

作者信息

Penyige A, Vargha G, Ensign J C, Barabás G

机构信息

Department of Biology, University Medical School, Debrecen, Hungary.

出版信息

Gene. 1992 Jun 15;115(1-2):181-5. doi: 10.1016/0378-1119(92)90557-6.

DOI:10.1016/0378-1119(92)90557-6

PMID:1612434

Abstract

The role of ADP ribosylation of proteins in the physiological regulation of sporulation in Streptomyces griseus was studied. We report here that both the activity of NAD+: arginine ADP-ribosyltransferase (ADPRT) and the pattern of ADP-ribosylated proteins showed characteristic changes during the life cycle in S. griseus 2682. Analysis off ADP-ribosylated proteins revealed that in a nonsporulating mutant of the parental wild-type (wt) strain (Bld7 mutant), both the activity of ADPRT and the pattern of ADP-ribosylated proteins were different from those of the parental strain. Addition of 3-aminobenzamide (3AB), the most potent inhibitor of ADPRT, inhibited sporulation of S. griseus 2682 and the A-factor (AF)-induced sporulation of S. griseus Bld7, but in both cases the inhibitory effect of 3AB was strictly age-dependent. Using [alpha-32P]GTP, we have demonstrated the presence of GTP-binding proteins in purified cell membranes of S. griseus 2682 and S. griseus Bld7. The same GTP-binding proteins were observed in Bld7 and the wt. AF stimulated the basal GTPase activity of cell membranes of S. griseus 2682 in a concentration-dependent manner, suggesting that GTP-binding proteins might be involved in the AF-induced sporulation process.

摘要

研究了蛋白质的 ADP 核糖基化在灰色链霉菌孢子形成生理调节中的作用。我们在此报告，在灰色链霉菌 2682 的生命周期中，NAD⁺：精氨酸 ADP 核糖基转移酶（ADPRT）的活性和 ADP 核糖基化蛋白质的模式均呈现出特征性变化。对 ADP 核糖基化蛋白质的分析表明，在亲本野生型（wt）菌株的非孢子形成突变体（Bld7 突变体）中，ADPRT 的活性和 ADP 核糖基化蛋白质的模式均与亲本菌株不同。添加 3-氨基苯甲酰胺（3AB），即 ADPRT 的最有效抑制剂，可抑制灰色链霉菌 2682 的孢子形成以及 A 因子（AF）诱导的灰色链霉菌 Bld7 的孢子形成，但在这两种情况下，3AB 的抑制作用都严格依赖于菌龄。使用[α-³²P]GTP，我们已证明在灰色链霉菌 2682 和灰色链霉菌 Bld7 的纯化细胞膜中存在 GTP 结合蛋白。在 Bld7 和野生型中观察到相同的 GTP 结合蛋白。AF 以浓度依赖的方式刺激灰色链霉菌 2682 细胞膜的基础 GTP 酶活性，这表明 GTP 结合蛋白可能参与 AF 诱导的孢子形成过程。