Yuan Qiang, Chen Zhi-Yun, Yan Mao-Xiang
Zhejiang College of Traditional Chinese Medicine, Hangzhou 310053, China.
Zhongguo Zhong Yao Za Zhi. 2005 Jun;30(12):933-5.
To observe the influences of FVP1 on both curative and negative effects of CTX.
The present study included two parts of experiments. In the part 1, 0.2 mL of 1 x 10(7) mL(-1) of S180 cells were inoculated in the subcutaneous layer of the right armpit of mice. All the mice were randomly divided into 3 groups: control group, in which mice were given with normal saline in 10 consecutive days, CTX group, in which mice were injected with 30 mg of CTX in the first and third days and saline in the other 8 days during the 10 consecutive days of treatment, and FVP1 and CTX group, in which the mice were injected with 30 mg x kg(-1) of CTX in the first and third days and FVP1 at 10 mg x kg(-1) in all 10 consecutive days of treatment. After above 10-day treatment , all the mice were killed and the tumor body was taken out and weighed to calculate the inhibiting rates on tumor. In the part 2 of experiments all the mice were divided into 3 groups: Normal control group, in which mice were not treated with any drugs, CTX-induced model group of inhibiting immune system, in which mice were injected with CTX at dose of 10 mg x kg(-1) in first two days and saline in the following 7 days; and small-, meddle-and large-dosage of FVP1 groups, in which mice were injected with CTX at the same dose as above in first two days and FVP1 intraperitoneally at 5, 10 and 20 mg x kg(-1) respectively in the following 7 days. CTX group was regarded as the control model. After the treatment, the peripheral white cells, thymus index, spleen index, the phagocytic power of macrophage of abdominal cavity, lymphocyte trastation rate and the activity of NK cell were detected.
(DFVP1 plus small dose of CTX obviously enhanced the inhibiting rate of CTX on tumor in the mice inoculated with S180 cells. (2) FVP1 at the different dose obviously antagozized CTX-induced leucopenia, atrophy, reduction of the phagocytic power of macrophage in abdominal cavity and restored the function of lymphocyte translation and the activity of NK cells.
FPV1 could enhance the curative effect of CTX in depressing tumor and attenuate the negative effect of CTX in inhibiting the function of immune system.
观察FVP1对环磷酰胺（CTX）疗效及不良反应的影响。
本研究包括两部分实验。在实验1中，将0.2 mL浓度为1×10⁷ mL⁻¹的S180细胞接种于小鼠右腋窝皮下。所有小鼠随机分为3组：对照组，连续10天给予生理盐水；CTX组，在连续10天的治疗中，第1天和第3天注射30 mg CTX，其余8天注射生理盐水；FVP1与CTX组，在连续10天的治疗中，第1天和第3天注射30 mg/kg CTX，且连续10天每天注射10 mg/kg FVP1。上述治疗10天后，处死所有小鼠，取出瘤体称重，计算肿瘤抑制率。在实验2中，所有小鼠分为3组：正常对照组，不给予任何药物处理；CTX诱导的免疫抑制模型组，前2天注射10 mg/kg CTX，后7天注射生理盐水；FVP1小、中、大剂量组，前2天注射与上述相同剂量的CTX，后7天分别腹腔注射5、10和20 mg/kg FVP1。CTX组作为对照模型。治疗后，检测外周血白细胞、胸腺指数、脾脏指数、腹腔巨噬细胞吞噬能力、淋巴细胞转化率及NK细胞活性。
（1）FVP1加小剂量CTX明显提高了接种S180细胞小鼠中CTX对肿瘤的抑制率。（2）不同剂量的FVP1明显拮抗CTX诱导的白细胞减少、胸腺萎缩、腹腔巨噬细胞吞噬能力降低，并恢复淋巴细胞转化功能及NK细胞活性。
FPV1可增强CTX的抑瘤疗效，并减轻CTX抑制免疫系统功能的不良反应。
