Li Mo-lin, Jia Yu-jie, Jiang Miao-na, Shu Xiao-hong, Li Chuan-gang
Pathophysiology Department of Dalian Medical University, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2008 Jun;24(6):567-9.
To establish a mouse model for BTT739 tumor-bearing mice cured by a low dose of cyclophosphamide (CTX). And then to observe the dynamic changes and significance of peripheral blood counts especially blood platelet count during tumor shrinkage induced by a low dose of CTX in T739 mice.
Mouse bladder carcinoma tissues were inoculated subcutaneously into T739 mice. Seven days later, different doses of CTX or the same volume of NS were administered intraperitoneally to treat these tumor-bearing T739 mice. Tumor sizes were observed and recorded subsequently to find out the minimal dose of CTX that could cure most of these tumor-bearing mice. Then another 12 tumor-bearing mice were randomly divided into 15 mg/kg CTX treatment group and control group. Blood samples were obtained from orbital venous sinus on different times after CTX treatment. Complete blood counts were performed and the relationship between peripheral blood platelet counts and tumor shrinkage was analyzed.
Within 2 weeks after CTX treatment, the speed of tumor shrinkage had a positive relationship with the dose of CTX used; but the survival rate of the tumor-bearing mice had a negative relationship with the dose of CTX used in 2 months after CTX treatment. 15 mg/kg CTX could cure most of the tumor bearing mice, while it had no remarkably inhibitive effects on peripheral blood cells. The perpherial platelet count increased to (1483.4+/-184.4)x10(9)/L in mice 6 h after CTX treatment. There was significant difference compared with that in mice of control group (1086.6+/-81.0)x10(9)/L (P<0.01). During the 2nd to 14th day after CTX treatment, there was no obvious difference in the platelet count between treatment group and control group (P>0.05).
CTX 15 mg/kg could cure most of bladder tumor-bearing T739 mice. The transient increase of the peripheral platelet count in 6 h after CTX treatment may relate to the antitumor effects of CTX.
建立低剂量环磷酰胺（CTX）治愈的BTT739荷瘤小鼠模型。进而观察低剂量CTX诱导T739小鼠肿瘤缩小过程中外周血细胞计数尤其是血小板计数的动态变化及意义。
将小鼠膀胱癌组织皮下接种于T739小鼠。7天后，对这些荷瘤T739小鼠腹腔注射不同剂量的CTX或相同体积的生理盐水进行治疗。随后观察并记录肿瘤大小，以找出能治愈大多数荷瘤小鼠的CTX最小剂量。然后将另外12只荷瘤小鼠随机分为15mg/kg CTX治疗组和对照组。在CTX治疗后的不同时间从眶静脉窦采集血样。进行全血细胞计数并分析外周血小板计数与肿瘤缩小之间的关系。
CTX治疗后2周内，肿瘤缩小速度与所用CTX剂量呈正相关；但在CTX治疗后2个月，荷瘤小鼠的生存率与所用CTX剂量呈负相关。15mg/kg CTX可治愈大多数荷瘤小鼠，而对外周血细胞无明显抑制作用。CTX治疗后6小时小鼠外周血小板计数升至(1483.4±184.4)×10⁹/L。与对照组小鼠(1086.6±81.0)×10⁹/L相比有显著差异（P<0.01）。在CTX治疗后的第2至14天，治疗组和对照组的血小板计数无明显差异（P>0.05）。
15mg/kg CTX可治愈大多数T739荷膀胱肿瘤小鼠。CTX治疗后6小时外周血小板计数的短暂升高可能与CTX的抗肿瘤作用有关。
