细胞质样品多次冻融循环对抗氧化酶活性的影响。
Effect of multiple freeze-thaw cycles of cytoplasm samples on the activity of antioxidant enzymes.
作者信息
Murias Marek, Rachtan Magdalena, Jodynis-Liebert Jadwiga
机构信息
Department of Toxicology, Poznan University of Medical Sciences, Dojazd 30, 60-631 Poznan, Poland.
出版信息
J Pharmacol Toxicol Methods. 2005 Sep-Oct;52(2):302-5. doi: 10.1016/j.vascn.2005.03.002.
INTRODUCTION
In this paper the activity of cytoplasmatic antioxidant enzymes after multiple freeze-thaw cycles of samples of cytoplasm was assayed in order to characterise the limits that would be necessary to impose on experimental design.
METHODS
The activities of antioxidant enzymes in samples of rat liver cytoplasm were evaluated after 1, 2, 3, 4, 5, 10 and 15 freeze-thaw cycles. The activity of antioxidant enzymes was determined by standard methods developed for this purpose.
RESULTS
The most resistant against freeze-thaw stress was catalase; even 15 freeze-thaw cycles did not change its activity. The remaining enzymes tested revealed a significant loss in their activity after 4-5 freeze-thaw cycles. The most sensitive was glutathione S-tranferase; its activity was significantly decreased after 3 thaw cycles.
DISCUSSION
The data presented may be helpful in designing experiments in which assay of antioxidant enzymes is a part.
引言
本文测定了细胞质样品经多次冻融循环后细胞质抗氧化酶的活性，以确定实验设计中所需的限制条件。
方法
对大鼠肝脏细胞质样品进行1、2、3、4、5、10和15次冻融循环后，评估抗氧化酶的活性。抗氧化酶的活性通过为此目的开发的标准方法测定。
结果
过氧化氢酶对冻融应激最具抗性；即使经过15次冻融循环，其活性也未改变。其余测试的酶在4-5次冻融循环后活性显著丧失。最敏感的是谷胱甘肽S-转移酶；其活性在3次解冻循环后显著降低。
讨论
所呈现的数据可能有助于设计以抗氧化酶测定为一部分的实验。
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