Balkan Burcu Menekse, Meral Ogunc, Cetintav Bekir, Tutun Hidayet, Ozkurt Guzin, Sel Tevhide
Department of Biochemistry, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, Turkey.
Department of Biochemistry, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey.
Vet Med Sci. 2025 Jul;11(4):e70418. doi: 10.1002/vms3.70418.
Accurate measurement of enzyme activities is very important in studies to evaluate enzymatic parameters. While performing these measurements, many factors can affect the results, including the method of obtaining the tissues, the conditions under which they are stored until analysis, and the methods of determining enzyme activity.
This study aimed to investigate the effect of different storage conditions (time and temperature) and different homogenisation buffers (PBS or KCl) on the enzymatic activity of superoxide dismutase (SOD), catalase (CAT), and adenosine deaminase (ADA) in homogenised cattle liver.
Fresh liver tissue samples were obtained from the slaughterhouse and homogenised in different homogenisation buffers. Supernatants from each sample were divided into three groups according to the experimental design of the study. SOD, CAT and ADA enzyme activities in homogenised tissues were evaluated.
Our data revealed that SOD, CAT and ADA activities did not differ significantly between PBS and KCl groups or between -20°C and -80°C freezing temperatures. However, our results showed that SOD levels decreased over time in both groups, CAT levels demonstrated a significant decrease from Month 0 to Month 3 and ADA levels decreased significantly over time.
The results indicated that storage time had a significant effect on enzyme activity changes, but the effect of storage temperature and homogenisation buffer was generally limited. These results may support the measurement of enzymatic activity in liver homogenate immediately and, if necessary, after freezing for the shortest possible time.
在评估酶参数的研究中，准确测量酶活性非常重要。在进行这些测量时，许多因素会影响结果，包括获取组织的方法、在分析前储存组织的条件以及测定酶活性的方法。
本研究旨在探讨不同储存条件（时间和温度）以及不同匀浆缓冲液（磷酸盐缓冲盐水或氯化钾）对匀浆牛肝中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和腺苷脱氨酶（ADA）酶活性的影响。
从屠宰场获取新鲜肝组织样本，并在不同的匀浆缓冲液中匀浆。根据研究的实验设计，将每个样本的上清液分为三组。评估匀浆组织中SOD、CAT和ADA的酶活性。
我们的数据显示，磷酸盐缓冲盐水组和氯化钾组之间，以及-20°C和-80°C冷冻温度之间，SOD、CAT和ADA活性没有显著差异。然而，我们的结果表明，两组中SOD水平均随时间下降，CAT水平从第0个月到第3个月显著下降，ADA水平随时间显著下降。
结果表明，储存时间对酶活性变化有显著影响，但储存温度和匀浆缓冲液的影响通常有限。这些结果可能支持立即测量肝匀浆中的酶活性，如有必要，在尽可能短的时间冷冻后进行测量。
