Thomas Biju B, Arai Shinichi, Ikai Yusuke, Qiu Guanting, Chen Zhenhai, Aramant Robert B, Sadda Srinivas R, Seiler Magdalene J
Department of Ophthalmology, Doheny Retina Institute, Keck School of Medicine, University of Southern California, 1450 San Pablo Street, Los Angeles, CA 90033, USA.
J Neurosci Methods. 2006 Mar 15;151(2):186-93. doi: 10.1016/j.jneumeth.2005.07.004. Epub 2005 Aug 29.
Optical coherence tomography (OCT), a non-invasive method, was used for qualitative assessment of fetal retinal sheet transplants by non-invasive imaging. Rhodopsin-mutant S334ter-line-3 rats with fast retinal degeneration (28-37-day old) were transplanted with fetal retinal sheets from embryonic day (E) 18-19 pigmented normal rats. Retinal thickness measurements from transplanted (n = 51), no surgery control (n = 8), and normal pigmented rat eyes (n = 6) were obtained using a Zeiss stratus OCT-3 scanning instrument. Frozen retinal sections were stained with hematoxylin/eosin. S334ter-line-3 rats showed significant reduction in OCT retinal thickness (p<0.001) compared to normal pigmented rats at the age of 21 days. In 62% of the transplanted rats, OCT scanning revealed the presence of a subretinal graft, which was confirmed by subsequent histology. Retinal thickness in the transplant area was significantly increased compared to the area outside the transplant and to non-transplanted eyes (p<0.001). While most of the transplants with single-band OCT images (87%) had rosetted transplants, a considerable proportion of transplants having a multi-band OCT image were found to have well-laminated areas in the graft after histological evaluation. Following retinal transplantation in rodents, OCT imaging data correlated mostly with transplant morphology. OCT is a useful technique for in vivo screening and evaluation of retinal transplants. This technique determines surgical outcomes at a much earlier stage.
光学相干断层扫描（OCT）是一种非侵入性方法，用于通过非侵入性成像对胎儿视网膜片移植进行定性评估。将快速视网膜变性的视紫红质突变型S334ter-3系大鼠（28 - 37日龄）移植来自胚胎第18 - 19天有色正常大鼠的胎儿视网膜片。使用蔡司Stratus OCT - 3扫描仪器获得移植大鼠（n = 51）、未手术对照大鼠（n = 8）和正常有色大鼠眼睛（n = 6）的视网膜厚度测量值。视网膜冷冻切片用苏木精/伊红染色。与21日龄的正常有色大鼠相比，S334ter-3系大鼠的OCT视网膜厚度显著降低（p<0.001）。在62%的移植大鼠中，OCT扫描显示存在视网膜下移植，随后的组织学检查证实了这一点。与移植区域外的区域和未移植的眼睛相比，移植区域的视网膜厚度显著增加（p<0.001）。虽然大多数单波段OCT图像的移植（87%）有玫瑰花结样移植，但在组织学评估后发现，相当一部分具有多波段OCT图像的移植在移植物中有良好分层区域。在啮齿动物进行视网膜移植后，OCT成像数据大多与移植形态相关。OCT是一种用于视网膜移植体内筛查和评估的有用技术。该技术能在更早阶段确定手术结果。
