Ramakrishnan S, Sulochana K N, Parikh S, Punitham R
Biochemistry Research Department, Vision Research Foundation, Chennai, India.
Indian J Ophthalmol. 1999 Mar;47(1):31-4.
To evaluate the presence of transthyretin (TTR, prealbumin) a protein which binds retinol to retinol-binding protein in various ocular tissues and to study its quantitative changes in the vitreous humor in various diseases.
Estimation of TTR was done by electrophoresis of 10 mg protein in each sample of tears, aqueous humor, vitreous, retina, and lens by an Imaging Densitometer using prealbumin as the standard.
TTR was present in all the eye structures except the lens and tear. The retina and the vitreous had relatively higher amounts of TTR compared with aqueous. The identity of TTR was confirmed by immuno-electrophoresis using anti-human TTR. Two bands in SDS electrophoresis revealed that this protein is a heterodimer. There was a significant decrease in vitreous TTR in diabetes with hypertension and increase in one case each of diabetes with hypertension associated with leukaemia or carcinoma with hepato-splenomegaly.
Vitreous TTR is probably from retina and retinal pigment epithelium. The level of vitreous TTR is likely to have diagnostic significance in some retinal diseases.
评估转甲状腺素蛋白（TTR，前白蛋白）的存在情况，该蛋白可将视黄醇与视黄醇结合蛋白结合，存在于各种眼组织中，并研究其在各种疾病的玻璃体液中的定量变化。
通过使用前白蛋白作为标准品的成像密度计，对每份眼泪、房水、玻璃体、视网膜和晶状体样本中的10mg蛋白质进行电泳，以估算TTR。
除晶状体和眼泪外，所有眼结构中均存在TTR。与房水相比，视网膜和玻璃体中的TTR含量相对较高。使用抗人TTR的免疫电泳证实了TTR的身份。SDS电泳中的两条带表明该蛋白是一种异二聚体。糖尿病合并高血压患者的玻璃体液TTR显著降低，而糖尿病合并高血压伴白血病或肝癌伴肝脾肿大的各1例患者中TTR升高。
玻璃体液TTR可能来自视网膜和视网膜色素上皮。玻璃体液TTR水平可能在某些视网膜疾病中具有诊断意义。
