Lüpke L, Distl O
Institute for Animal Breeding and Genetics, University of Veterinary Medicine Hannover, Hannover, Germany.
J Anim Breed Genet. 2005 Apr;122(2):131-9. doi: 10.1111/j.1439-0388.2005.00501.x.
Genetic variability of the dog breed Hanoverian Hound was analysed using a set of 16 microsatellites. The sample of 92 dogs was representative for the total current population [n=334, inbreeding coefficient 9.2%, relationship coefficient 11.2%] with respect to the level and distribution of the inbreeding and relationship coefficients. All microsatellites used were in Hardy-Weinberg equilibrium. The average number of alleles was 6.4. The average observed heterozygosity (H(O)) was slightly higher than the expected heterozygosity (H(E)). Dinucleotide microsatellites exhibited lower polymorphism information content (PIC) than tetranucleotide microsatellites (0.52 versus 0.66). The average PIC was 0.61. The individual inbreeding coefficient was negatively related to the average H(O) of all microsatellites, whereas the proportion of genes from introducing of Hanoverian Hounds from abroad showed no relationships to H(O). We found that the genetic variability in the Hanoverian Hounds analysed here was unexpectedly higher than that previously published for dog breeds of similar population size. Even in dog breeds of larger population size heterogyzosity was seldom higher than that observed here. The rather high genetic variability as quantified by polymorphic microsatellites in Hanoverian Hounds may be due to a large genetic variation in the founder animals of this breed and to the fact that this genetic diversity could be maintained despite genetic bottlenecks experienced by this breed in the 1920s and 1950s and despite the presence of high inbreeding and relationship coefficients for more than 50 years.
利用一组16个微卫星对汉诺威猎犬品种的遗传变异性进行了分析。92只狗的样本在近亲繁殖系数和亲缘系数的水平及分布方面代表了当前的整个种群[n = 334，近亲繁殖系数9.2%，亲缘系数11.2%]。所使用的所有微卫星均处于哈迪-温伯格平衡状态。等位基因的平均数量为6.4。平均观察杂合度（H(O)）略高于预期杂合度（H(E)）。二核苷酸微卫星显示出比四核苷酸微卫星更低的多态性信息含量（PIC）（0.52对0.66）。平均PIC为0.61。个体近亲繁殖系数与所有微卫星的平均H(O)呈负相关，而从国外引进汉诺威猎犬的基因比例与H(O)没有关系。我们发现，这里分析的汉诺威猎犬的遗传变异性出乎意料地高于之前发表的类似种群规模的犬种。即使在种群规模较大的犬种中，杂合度也很少高于这里观察到的水平。汉诺威猎犬中由多态微卫星量化的相当高的遗传变异性可能是由于该品种奠基动物的大量遗传变异，以及尽管该品种在20世纪20年代和50年代经历了遗传瓶颈，并且尽管存在超过50年的高近亲繁殖系数和亲缘系数，但这种遗传多样性仍得以维持。
