快速分离人补体成分C9以验证溶血C9微量测定法的特异性。

Rapid isolation of human complement component C9 to verify the specificity of a haemolytic C9 microassay.

作者信息

Bootsma H J, van den Berg C W, van Dijk H

机构信息

Eijkman-Winkler Laboratory of Medical Microbiology, University Hospital, Utrecht, Netherlands.

出版信息

J Chromatogr. 1992 Feb 7;591(1-2):187-93. doi: 10.1016/0021-9673(92)80237-o.

DOI:10.1016/0021-9673(92)80237-o

PMID:1613052

Abstract

A sensitive, haemolytic microassay of human complement component C9 was developed. The assay is based on the principle of reactive (C5b6-initiated) haemolysis and uses commercially available C9-depleted serum as reagent for C9. The specificity of the assay was verified by rapid, activity-guided isolation of the haemolytic component from human serum using high-performance liquid chromatography (HPLC) on a system for fast protein liquid chromatography. This isolation yielded a single component with characteristics of C9. The results suggest that rapid, activity-guided isolation as a new application of HPLC can be a useful tool to demonstrate the specificity of a functional assay.

摘要

开发了一种灵敏的人补体成分C9溶血微量测定法。该测定法基于反应性（C5b6启动）溶血原理，并使用市售的C9缺失血清作为C9的试剂。通过在快速蛋白质液相色谱系统上使用高效液相色谱（HPLC）从人血清中快速、活性导向分离溶血成分，验证了该测定法的特异性。这种分离产生了一种具有C9特征的单一成分。结果表明，快速、活性导向分离作为HPLC的一种新应用，可成为证明功能测定法特异性的有用工具。