Arvidsson E, Jansson S O, Schill G
Analytical Chemistry, Astra Hässle AB, Mölndal, Sweden.
J Chromatogr. 1992 Feb 7;591(1-2):55-63. doi: 10.1016/0021-9673(92)80222-g.
Stereoselective separations of charged enantiomers on CHIRAL-AGP can be controlled by varying the pH and adding charged and uncharged additives to the mobile phase. The interaction with the selector, alpha 1-acid glycoprotein, was studied by monitoring the effects of the variables on retention and by indirect detection, in part using a simple multivariate design. The stereoselectivity is due to simultaneous retention processes involving ion-exchange and ion-pairing mechanisms. The predominant mode of interaction for different solutes was elucidated from variables that promote or counteract either of the processes. Considerable improvements in the stereoselectivity were achieved with chiral or achiral anionic and cationic additives that act in a synergistic or competitive mode.
在手性α1-酸性糖蛋白上对带电对映体进行立体选择性分离，可以通过改变pH值以及向流动相中添加带电和不带电添加剂来控制。通过监测这些变量对保留的影响并采用间接检测方法，部分采用简单的多变量设计，研究了与选择剂α1-酸性糖蛋白的相互作用。立体选择性是由于同时存在涉及离子交换和离子对机制的保留过程。从促进或抵消任一过程的变量中阐明了不同溶质的主要相互作用模式。使用以协同或竞争模式起作用的手性或非手性阴离子和阳离子添加剂，可实现立体选择性的显著提高。
