Rand-Weaver M, Swanson P, Kawauchi H, Dickhoff W W
Laboratory of Molecular Endocrinology, School of Fisheries Sciences, Kitasato University, Iwate, Japan.
J Endocrinol. 1992 Jun;133(3):393-403. doi: 10.1677/joe.0.1330393.
Somatolactin (SL), a newly discovered fish pituitary protein belonging to the GH/prolactin family, was isolated from coho salmon (Oncorhynchus kisutch). Antibodies were raised to purified coho SL, and a homologous radioimmunoassay was developed and validated. The assay was specific for SL as indicated by the absence of cross-reactivity with coho salmon GH, gonadotrophins I and II and less than 0.2% cross-reaction to prolactin. Serial dilutions of plasma and pituitary extracts from Oncorhynchus species including coho salmon, chinook salmon and rainbow trout were parallel to the coho salmon SL standard curve. Displacement curves for dilutions of Atlantic salmon (Salmo salar) plasma, but not pituitary extract were parallel to the standards. Plasma levels of SL were measured in coho salmon throughout the final year of reproductive maturation. During the period of gonadal growth, plasma SL levels increased and were highly correlated to oestradiol levels in females and 11-ketotestosterone levels in males. Peak levels of SL were observed at the time of final maturation and spawning in both sexes. It is hypothesized that SL may regulate some physiological aspect of reproduction.
生长抑素（SL）是一种新发现的鱼类垂体蛋白，属于生长激素/催乳素家族，从银大麻哈鱼（Oncorhynchus kisutch）中分离得到。制备了针对纯化的银大麻哈鱼SL的抗体，并开发和验证了一种同源放射免疫测定法。该测定法对SL具有特异性，与银大麻哈鱼生长激素、促性腺激素I和II无交叉反应，与催乳素的交叉反应小于0.2%，证明了这一点。包括银大麻哈鱼、大鳞大麻哈鱼和虹鳟在内的大麻哈鱼属物种的血浆和垂体提取物的系列稀释液与银大麻哈鱼SL标准曲线平行。大西洋鲑（Salmo salar）血浆稀释液的置换曲线与标准曲线平行，但垂体提取物的置换曲线不平行。在生殖成熟的最后一年，对银大麻哈鱼的血浆SL水平进行了测量。在性腺生长期间，血浆SL水平升高，与雌性的雌二醇水平和雄性的11-酮睾酮水平高度相关。在两性最终成熟和产卵时观察到SL的峰值水平。据推测，SL可能调节繁殖的某些生理方面。
