Bostwick J R, Abbe R, Appel S H
Department of Neurology, Baylor College of Medicine, Houston, Texas 77030.
J Neurochem. 1992 Jul;59(1):236-44. doi: 10.1111/j.1471-4159.1992.tb08896.x.
Dissociated rat septal nucleus cells cultured in defined medium exhibited twofold increases in the maximal rates of sodium-dependent, high-affinity choline uptake and acetylcholine formation when grown in the presence of phosphoethanolamine. The effect was concentration-dependent (EC50 = 15 microM) and appeared to be associated with in vitro maturation of cholinergic neurons rather than with enhanced survival. Choline acetyltransferase, acetylcholinesterase, and choline kinase activities were unaffected by this treatment. The effect of phosphoethanolamine was specific for cholinergic neurons, because treatment with this compound did not alter the kinetic constants for high-affinity neuronal uptake of gamma-aminobutyric acid or dopamine. The action appeared to be mediated primarily through activation of the sodium-dependent, high-affinity transport mechanism for choline as opposed to alterations in the storage and release of acetylcholine.
在限定培养基中培养的解离大鼠隔核细胞，当在磷酸乙醇胺存在下生长时，钠依赖性高亲和力胆碱摄取和乙酰胆碱形成的最大速率增加了两倍。该效应呈浓度依赖性（EC50 = 15微摩尔），似乎与胆碱能神经元的体外成熟有关，而不是与存活率提高有关。胆碱乙酰转移酶、乙酰胆碱酯酶和胆碱激酶活性不受该处理的影响。磷酸乙醇胺的作用对胆碱能神经元具有特异性，因为用该化合物处理不会改变γ-氨基丁酸或多巴胺的高亲和力神经元摄取的动力学常数。该作用似乎主要是通过激活胆碱的钠依赖性高亲和力转运机制介导的，而不是通过乙酰胆碱的储存和释放的改变。
