Park Sang Mi, Lee Jung Suk, Jegal Sung, Jeon Bo Young, Jung Min, Park Yoon Sik, Han Sang Lyul, Shin Yoon Sup, Her Nam Han, Lee Jang Ha, Lee Mi Yeon, Ryu Ki Hyun, Yang Seung Gyun, Harn Chee Hark
Biotechnology Center, Nong Woo Bio, Jeongdan, Ganam, Yeoju, Gyeonggi, 469-884, Korea.
Plant Cell Rep. 2005 Aug;24(6):350-6. doi: 10.1007/s00299-005-0946-8. Epub 2005 Jun 14.
In watermelon, grafting of seedlings to rootstocks is necessary because watermelon roots are less viable than the rootstock. Moreover, commercially important watermelon varieties require disease-resistant rootstocks to reduce total watermelon yield losses due to infection with viruses such as cucumber green mottle mosaic virus (CGMMV). Therefore, we undertook to develop a CGMMV-resistant watermelon rootstock using a cDNA encoding the CGMMV coat protein gene (CGMMV-CP), and successfully transformed a watermelon rootstock named 'gongdae'. The transformation rate was as low as 0.1-0.3%, depending on the transformation method used (ordinary co-culture vs injection, respectively). However, watermelon transformation was reproducibly and reliably achieved using these two methods. Southern blot analysis confirmed that the CGMMV-CP gene was inserted into different locations in the genome either singly or multiple copies. Resistance testing against CGMMV showed that 10 plants among 140 T1 plants were resistant to CGMMV infection. This is the first report of the development by genetic engineering of watermelons resistant to CGMMV infection.
在西瓜种植中，将幼苗嫁接到砧木上是必要的，因为西瓜根系的活力不如砧木。此外，具有商业重要性的西瓜品种需要抗病砧木，以减少因感染黄瓜绿斑驳花叶病毒（CGMMV）等病毒而导致的西瓜总产量损失。因此，我们着手利用编码CGMMV外壳蛋白基因（CGMMV-CP）的cDNA开发一种抗CGMMV的西瓜砧木，并成功转化了一种名为“公大”的西瓜砧木。根据所使用的转化方法（分别为普通共培养法和注射法），转化率低至0.1-0.3%。然而，使用这两种方法都能可重复且可靠地实现西瓜转化。Southern杂交分析证实，CGMMV-CP基因以单拷贝或多拷贝的形式插入到基因组的不同位置。对CGMMV的抗性测试表明，140株T1代植株中有10株对CGMMV感染具有抗性。这是关于通过基因工程培育抗CGMMV感染西瓜的首次报道。
