Liu Ying, Yuan Can, Zhang Hua-Li
Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2004 Feb;29(1):1-5.
To screen select target genes regulated by heat shock transcription factor 1 (HSF1 ) in heat shock response from myocardium of HSF1 gene knockout mice using cDNA chip.
HSF1 knockout mice were subjected to heat shock treatment (42 degrees C for 15 minutes, and recovered for 3 hours), and cDNA chip containing 15 000 mouse cDNAs were employed to investigate the expression of genes regulated by HSF1 in the myocardium. Then 4 of the differentially expressed genes in the myocardium of HSF1 knockout mice were further confirmed by RT-PCR. The promoter regions of the differentially expressed genes were analysed by TESS software to find HSE.
Three hundred and ninety-eight genes were down - regulated in the myocardium of HSF1 knockout mice in heat shock response,with 173 known. Whereas 641 genes were up-regulated in the myocardium, with 235 known. The 5 down-regulated genes and 6 up-regulated ones were shown to contain HSE in their promoter regions.
In heat shock response, expression of multiple genes is regulated directly or indirectly by HSF1.
利用cDNA芯片从热休克转录因子1（HSF1）基因敲除小鼠心肌中筛选出热休克反应中受HSF1调控的靶基因。
对HSF1基因敲除小鼠进行热休克处理（42℃，15分钟，恢复3小时），采用包含15000个小鼠cDNA的cDNA芯片研究HSF1调控的心肌基因表达。然后通过RT-PCR进一步确认HSF1基因敲除小鼠心肌中4个差异表达基因。利用TESS软件分析差异表达基因的启动子区域以寻找热休克元件（HSE）。
在热休克反应中，HSF1基因敲除小鼠心肌中有398个基因表达下调，其中173个为已知基因。而心肌中有641个基因表达上调，其中235个为已知基因。5个下调基因和6个上调基因在其启动子区域含有热休克元件。
在热休克反应中，多个基因的表达直接或间接受HSF1调控。
