[Mechanisms of heat shock proteins inhibiting C2C12 cell apoptosis induced by hydrogen peroxide].

OBJECTIVE

To explore the mechanisms of C2C12 cell apoptosis induced by hydrogen peroxide (H2O2) which is inhibited by heat shock proteins.

METHODS

The expression of heat shock proteins in mouse embryonic myogenic cell line, C2C12 cell,was induced by heat shock response. C2C12 cell apoptosis induced by 0.5 mmol/L hydrogen peroxide (H2O2) was determined by Hoechst 33258 staining. The activities of caspase -3,8,9 were assayed by caspase colorimetric assay kit and Western-blotting. The release of cytochrome C from mitochondria was observed by Western-blotting of cell mitochondria and cytosol fractions.

RESULTS

The expression of HSP70 and alphaB-crystallin in C2C12 cell significantly increased at 24 hour after the heat shock response. Heat shock response could inhibit the release of cytochrome c from mitochondria to cytoplasm,the activation of caspase -3,8,9 and the subsequent apoptosis induced by H2O2 in C2C12 cell.

CONCLUSION

HSPs can inhibit the C2C12 cell apoptosis through interference with the activation of both mitochondrial and death receptor pathways, which can provide new clues for the prevention of cardiovascular diseases.