Tan De-Ming, Yang Yong-Feng, Liu Shui-Ping
Institute of Infectious Disease, Xiangya Hospital, Central South University, Changsha 410008, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2004 Feb;29(1):47-9.
To investigate the effect of interleukin-12 (IL-12) on immune responses induced by pcDNA3 which expresses hepatitis B virus pan-S gene.
BABL/c mice were immunized with pcDNA3 alone (control group), pcDNA3-PS alone, or co-immunized with pcDNA3-PS and pcDNA3-IL12. Six weeks after the immunization, sero anti-HBS antibody was examined, lymphocyte proliferation test and specific cytokine level were assayed. RESULTS The titer of anti-HBs antibody, the stimulation index of splenocytes, and cytokine level of IL-12 and IFN-gamma from the mice coimmunized with pcDNA3-PS and pcDNA3-IL12 were significantly higher than those of the mice immunized alone with pcDNA3-PS. Antibody level, SI, and cytokine level of the immunized mice were higher than those of the control group.
IL-12 expression plasmid can act as immunological adjuvants for HBV pan-S gene vaccine.
研究白细胞介素-12（IL-12）对表达乙肝病毒全S基因的pcDNA3诱导的免疫反应的影响。
将BABL/c小鼠分别用单独的pcDNA3（对照组）、单独的pcDNA3-PS或pcDNA3-PS与pcDNA3-IL12联合免疫。免疫六周后，检测血清抗-HBS抗体，进行淋巴细胞增殖试验并测定特异性细胞因子水平。结果：与单独用pcDNA3-PS免疫的小鼠相比，用pcDNA3-PS和pcDNA3-IL12联合免疫的小鼠的抗-HBs抗体滴度、脾细胞刺激指数以及IL-12和IFN-γ的细胞因子水平显著更高。免疫小鼠的抗体水平、SI和细胞因子水平高于对照组。
IL-12表达质粒可作为乙肝病毒全S基因疫苗的免疫佐剂。
