Goto S, Kobayashi M, Hidaka H, Saito H
First Department of Internal Medicine, Nagoya University School of Medicine.
Nihon Rinsho. 1992 Feb;50(2):214-7.
We investigated the intracellular processes of the shape change in the human megakaryoblastic leukemia cell, MEG-01, by platelet agonists. Thrombin induced the formation of many pseudopods. This shape change was also induced by TPA and A23187, but not by ADP, collagen, or epinephrine. Electron microscopy and FITC-labeled phalloidin staining revealed thick submembranous microfilament bundles in the pseudopods of the shape-changed cells induced by thrombin. Shape change was inhibited by cytochalasin B. Protein kinase C (RKC) inhibitor, H-7, markedly inhibited thrombin-induced shape change, while the myosin light chain kinase (MLCK) inhibitor, ML-9 did not. These results suggest that thrombin-induced reorganization of microfilaments and shape change of MEG-01 cells are mediated by PKC but not by MLCK.
我们研究了血小板激动剂对人巨核母细胞白血病细胞MEG - 01形状变化的细胞内过程。凝血酶诱导形成许多伪足。这种形状变化也可由佛波酯（TPA）和A23187诱导，但ADP、胶原蛋白或肾上腺素不能诱导。电子显微镜和异硫氰酸荧光素（FITC）标记的鬼笔环肽染色显示，在凝血酶诱导形状改变的细胞伪足中有厚厚的膜下微丝束。细胞松弛素B可抑制形状变化。蛋白激酶C（PKC）抑制剂H - 7显著抑制凝血酶诱导的形状变化，而肌球蛋白轻链激酶（MLCK）抑制剂ML - 9则无此作用。这些结果表明，凝血酶诱导的微丝重组和MEG - 01细胞的形状变化是由PKC介导的，而非由MLCK介导。
