Berman Jason N, Kanki John P, Look A Thomas
Department of Pediatric Oncology, Dana-Farber Cancer Institute and Harvard Medical School, Boston, Mass. 02115, USA.
Exp Hematol. 2005 Sep;33(9):997-1006. doi: 10.1016/j.exphem.2005.06.010.
Zebrafish produce nearly identical hematopoeitic cell lineages to those found in mammals and other higher vertebrates. As in mammals, blood cell development proceeds in distinct waves, constituting embryonic (primitive) and adult (definitive) hematopoiesis. The conservation of genes such as scl, pu.1, c/ebpalpha, mpo, l-plastin, and lysozyme C in myelopoiesis and the corresponding expression patterns in zebrafish suggests that shared genetic pathways regulate this complex developmental process. In the zebrafish model system, experimental approaches have been applied, including RNA in situ hybridization, morpholino injections, and the analysis of mutant and transgenic fish lines, leading to improved understanding of the regulation in vivo of key molecular pathways with conserved roles in vertebrate myelopoiesis.
斑马鱼产生的造血细胞谱系与哺乳动物和其他高等脊椎动物中发现的几乎完全相同。与哺乳动物一样，血细胞发育以不同的阶段进行，包括胚胎期（原始）造血和成年期（定型）造血。髓系造血中scl、pu.1、c/ebpα、mpo、l-原肌球蛋白和溶菌酶C等基因的保守性以及在斑马鱼中的相应表达模式表明，共享的遗传途径调节这一复杂的发育过程。在斑马鱼模型系统中，已经应用了多种实验方法，包括RNA原位杂交、吗啉代注射以及对突变体和转基因鱼系的分析，从而增进了对在脊椎动物髓系造血中具有保守作用的关键分子途径体内调节的理解。
