Balic Marija, Dandachi Nadia, Hofmann Günter, Samonigg Hellmut, Loibner Hans, Obwaller Andreas, van der Kooi Alexander, Tibbe Arjan G J, Doyle Gerald V, Terstappen Leon W M M, Bauernhofer Thomas
Department of Clinical Oncology, University Hospital Graz, Austria.
Cytometry B Clin Cytom. 2005 Nov;68(1):25-30. doi: 10.1002/cyto.b.20065.
Monitoring of circulating tumor cells (CTCs) in blood of carcinoma patients treated with novel compounds may be a measurement of treatment effectiveness. Before it can be used clinically, a reliably method is needed to enumerate CTCs. We compared two methods for CTC enumeration, OnkoQuick and the CellSearch system.
We drew 22.5 ml of blood into three CellSave tubes from 15 healthy donors and 61 patients with metastatic carcinoma. After pooling, 15 ml was processed with OncoQuick and 7.5 ml with CellSearch.
With both methods no CTCs were found in healthy donors. At least one CTC was detected in 14 of 61 patients (23%) with OncoQuick and 33 of 61 patients (54%) with CellSearch (P < 0.0001). The number of CTCs detected was larger for CellSearch (mean 20 CTCs/7.5 ml of blood) than for OncoQuick (3 CTCs/7.5 ml; P < 0.0001).
The CellSearch system is a more accurate and sensitive method to enumerate CTCs. Further studies are warranted to evaluate CTC enumeration by the CellSearch system as a monitoring tool for the evaluation of the efficacy of novel anticancer agents.
监测接受新型化合物治疗的癌症患者血液中的循环肿瘤细胞（CTC）可能是衡量治疗效果的一种方法。在其能够用于临床之前，需要一种可靠的方法来计数CTC。我们比较了两种计数CTC的方法，即OnkoQuick法和CellSearch系统。
我们从15名健康供者和61名转移性癌患者中采集22.5毫升血液，注入三支CellSave管中。混合后，15毫升血液用OnkoQuick法处理，7.5毫升血液用CellSearch系统处理。
两种方法在健康供者中均未检测到CTC。OnkoQuick法在61名患者中的14名（23%）检测到至少一个CTC，CellSearch系统在61名患者中的33名（54%）检测到至少一个CTC（P<0.0001）。CellSearch系统检测到的CTC数量（平均每7.5毫升血液20个CTC）多于OnkoQuick法（每7.5毫升血液3个CTC；P<0.0001）。
CellSearch系统是一种更准确、更灵敏的计数CTC的方法。有必要进一步研究评估将CellSearch系统计数CTC作为评估新型抗癌药物疗效的监测工具。
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