Resel Folkersma L, Olivier Gómez C, San José Manso L, Veganzones de Castro S, Galante Romo I, Vidaurreta Lázaro M, de la Orden G V, Arroyo Fernández M, Díaz Rubio E, Silmi Moyano A, Maestro de Las Casas M A
Urology Department, Análisis Clínicos Department, Servicio de Oncologic Department, Hospital Universitario Clínico San Carlos, Madrid, Spain.
Arch Esp Urol. 2010 Jan-Feb;63(1):23-31.
To detect and enumerate circulating prostatic tumor cells (CTC) in the peripheral blood of patients with prostate cancer (PC) and study the relationship between CTCs and clinical-pathological parameters.
Prospective three-arm study: 26 patients (p) with localised PC (LPC); 24 P with metastatic PC (MPC) and 30 healthy volunteer controls. A single 7.5 ml sample of peripheral blood was retrieved; CTCs were isolated using an immunomagnetic method based on the CellSearch system (Veridex). CTCs were identified as nucleated cells negative for CD45 (leukocytes) and positive for cytokeratins. (8, 18 y 19) The relationship between CTC numbers and PSA levels, Gleason score and TNM classification was studied.
Only 10% of the healthy controls had 1 CTC/7.5 mL, none of the patients with localised PC had more than 3 CTCs (88% < or = 2 CTCs), and patients with MPC had significantly higher CTC levels [m: 29 (1-178)] compared with the other two groups (P: 0.000). A positive correlation was demonstrated between the CTC count and PSA levels, tumor size, and presence or absence of enlarged lymph nodes. Gleason score was the only parameter that did not show any correlation with CTC levels, and although the number of CTCs was higher in patients with visceral metastases [m: 297 (0-416)] compared with bone metastases patients [m: 68 (9.5-168)] , these differences were not significant.
Immunomagnetic analysis permits CTCs to be enumerated in peripheral blood and could be a possible way to correctly stage and make a reasonable prognosis of metastatic disease.
检测并计数前列腺癌（PC）患者外周血中循环前列腺肿瘤细胞（CTC），并研究CTC与临床病理参数之间的关系。
前瞻性三臂研究：26例局限性前列腺癌（LPC）患者；24例转移性前列腺癌（MPC）患者和30名健康志愿者作为对照。采集7.5 ml外周血样本；使用基于CellSearch系统（Veridex）的免疫磁珠法分离CTC。CTC被鉴定为CD45阴性（白细胞）且细胞角蛋白阳性的有核细胞。（8、18和19）研究CTC数量与前列腺特异性抗原（PSA）水平、 Gleason评分和TNM分期之间的关系。
仅10%的健康对照者每7.5 ml血液中有1个CTC，局限性前列腺癌患者均无超过3个CTC（88%≤2个CTC），转移性前列腺癌患者的CTC水平显著高于其他两组[m：29（1 - 178）]（P：0.000）。CTC计数与PSA水平、肿瘤大小以及有无肿大淋巴结之间呈正相关。Gleason评分是唯一与CTC水平无相关性的参数，尽管内脏转移患者的CTC数量高于骨转移患者[m：297（0 - 416）]与[m：68（9.5 - 168）]，但这些差异无统计学意义。
免疫磁珠分析可对外周血中的CTC进行计数，可能是对转移性疾病进行准确分期和合理预后评估的一种方法。
