Kang Ung Gu, Jeon Won Je, Kim Yeni, Chung Chun Kee, Park Joo Bae, Juhnn Yong Sung, Kim Yong Sik
Department of Psychiatry & Behavioral Science, Seoul National University College of Medicine, Seoul, South Korea.
Neurosci Lett. 2005 Dec 30;390(3):171-5. doi: 10.1016/j.neulet.2005.08.020. Epub 2005 Sep 6.
We have attempted to determine the effects of electroconvulsive shock (ECS) on protein phosphatase 2A (PP2A) in the frontal cortices of rats. PP2A exhibited a 30% increase in activity immediately after ECS treatment. Immunoblot analysis revealed that phosphorylation signals, including protein kinase B (Akt/PKB), glycogen synthase kinase-3beta (GSK-3beta), and cyclic adenosine monophosphate response element binding protein (CREB) were reduced immediately after ECS treatment. When an additional ECS was administered after the activation of these kinases, the immediate reactivation of PP2A overrode the kinase activity. ECS induces transient PP2A activation prior to kinase activation, and this pattern of activity may induce the biphasic phosphorylation of substrate proteins.
我们试图确定电休克（ECS）对大鼠额叶皮质中蛋白磷酸酶2A（PP2A）的影响。ECS治疗后，PP2A的活性立即增加了30%。免疫印迹分析显示，包括蛋白激酶B（Akt/PKB）、糖原合酶激酶-3β（GSK-3β）和环磷酸腺苷反应元件结合蛋白（CREB）在内的磷酸化信号在ECS治疗后立即减少。当在这些激酶激活后再给予一次ECS时，PP2A的立即重新激活超过了激酶活性。ECS在激酶激活之前诱导PP2A瞬时激活，这种活性模式可能诱导底物蛋白的双相磷酸化。
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