Van Hoof Nathalie, Courtheyn Dirk, Antignac Jean-Philippe, Van de Wiele Mieke, Poelmans Sofie, Noppe Herlinde, De Brabander Hubert
Laboratory of Chemical Analysis, Ghent University, Department of Veterinary Public Health and Food Safety, Salisburylaan 133, B-9820 Merelbeke, Belgium.
Rapid Commun Mass Spectrom. 2005;19(19):2801-8. doi: 10.1002/rcm.2126.
Ion suppression, a matrix effect that affects quantitative mass spectrometry, is one of the main problems encountered in liquid chromatography/tandem mass spectrometry. Two different clean-up steps for the multi-residue analysis of beta-agonists in urine were evaluated with respect to minimisation of ion suppression, namely, a mixed-phase solid phase extraction (SPE) column, i.e., clean screen Dau (CSD), and a molecular imprinted polymer (MIP) SPE column. Ion suppression experiments revealed that CSD sample clean-up can lead to false negative results for some beta-agonists, and that clean-up using MIP columns is more selective for beta-agonists than the use of CSD columns.
