Bujía J, Pitzke P, Wilmes E, Hammer C
Department of Otolaryngology, Ludwig-Maximilians-Universität München, FRG.
ORL J Otorhinolaryngol Relat Spec. 1992;54(2):80-4. doi: 10.1159/000276267.
One of the reasons for failure of cartilage allografts is the impaired condition of the transplant during storage. In this paper we describe methods for the isolation and culture of viable chondrocytes obtained from nasal septum cartilage. Furthermore, we evaluate the possibility of growing such specific chondrocytes under culture conditions and storing them in a frozen state. Age-dependent differences were observed in the growth rate of the cultured cells. Our results confirm that chondrocytes survive freezing and remain able to proliferate. Knowledge gained from this study may be applied to the culture and freezing of viable intact cartilage for use in reconstructive surgery in otolaryngology.
同种异体软骨移植失败的原因之一是移植体在储存期间的受损状况。在本文中，我们描述了从鼻中隔软骨中获取活软骨细胞并进行分离和培养的方法。此外，我们评估了在培养条件下培养此类特定软骨细胞并将其冷冻保存的可能性。在培养细胞的生长速率方面观察到了年龄依赖性差异。我们的结果证实软骨细胞在冷冻后存活并仍能增殖。从这项研究中获得的知识可应用于用于耳鼻喉科重建手术的活完整软骨的培养和冷冻。
