Wee J L, Ho L C, Yap E H, Singh M
Department of Microbiology, National University of Singapore.
Parasitology. 1992 Feb;104 Pt 1:25-31. doi: 10.1017/s0031182000060765.
A murine monoclonal antibody which reacts to 22 and 41 kDa Toxoplasma gondii surface antigens was employed in an IgM capture enzyme-linked immunosorbent assay (ELISA). A total of 125 patients' sera were tested in the monoclonal-based assay. When compared with a commercial ELISA test (Abbott Toxo-M EIA) which uses polyclonal anti-T. gondii antibodies, good correlation (Pearsons coefficient r = 0.91) was observed. The specificity of the assay was studied by testing a panel of control sera obtained from healthy individuals and blood transfusion donors; all sera gave negative results. Serum samples positive for T. gondii antibodies were treated with 2-mercaptoethanol (2-ME) to demonstrate the specificity of the test for IgM antibodies. Reactivity of these sera was lost after the treatment. The test is not subject to interference by rheumatoid factor as sera positive for rheumatoid factor were negative in the assay. Reproducibility was good with the coefficients of variation for within-day tests below 10% and not exceeding 18% for day-to-day tests. The monoclonal-based assay is simple to perform and appears to be a viable test for diagnosis of T. gondii infection.
一种与22 kDa和41 kDa刚地弓形虫表面抗原发生反应的鼠单克隆抗体被用于IgM捕获酶联免疫吸附测定（ELISA）。总共125份患者血清在基于单克隆抗体的检测中进行了检测。与使用多克隆抗刚地弓形虫抗体的商业ELISA检测（雅培弓形虫酶免疫测定）相比，观察到良好的相关性（皮尔逊系数r = 0.91）。通过检测一组从健康个体和输血者获得的对照血清来研究该检测的特异性；所有血清均给出阴性结果。用2-巯基乙醇（2-ME）处理刚地弓形虫抗体阳性的血清样本，以证明该检测对IgM抗体的特异性。处理后这些血清的反应性丧失。该检测不受类风湿因子的干扰，因为类风湿因子阳性的血清在检测中呈阴性。日内检测的变异系数低于10%，日间检测不超过18%，重复性良好。基于单克隆抗体的检测操作简单，似乎是诊断刚地弓形虫感染的一种可行检测方法。
