质粒ColIb-P9基本复制子中顺式作用序列的结构和功能特征
Structural and functional features of cis-acting sequences in the basic replicon of plasmid ColIb-P9.
作者信息
Tanaka K, Sakai H, Honda Y, Nakamura T, Higashi A, Komano T
机构信息
Department of Agricultural Chemistry, Kyoto University, Japan.
出版信息
Nucleic Acids Res. 1992 Jun 11;20(11):2705-10. doi: 10.1093/nar/20.11.2705.
Abstract
We have structurally and functionally analyzed the cis-elements essential for ColIb-P9 plasmid DNA replication. The putative oriV region encompassed a region of 172 base pairs (bp) located 152 bp downstream of the repZ gene. A typical dnaA box found in this region proved nonessential for the DNA replication of ColIb-P9. The ssi signal of ColIb-P9 is a homologue of the G-sites of R1 and R100 plasmids. Deletion of the G-site led to 1.5-fold reduction of the copy number, suggesting that although this G-site is not essential, it is important for efficient ColIb-P9 DNA replication. In addition, the ColIb-P9 replicon is highly and extensively homologous with the P307 (RepFIC) replicon, and highly homologous with the R100 (RepFIIA) replicon around the G-site region. These facts imply a common ancestry from which the plasmids have evolved.
摘要
我们已经对ColIb-P9质粒DNA复制所必需的顺式元件进行了结构和功能分析。假定的oriV区域包含一个位于repZ基因下游152 bp处的172个碱基对（bp）的区域。在该区域发现的典型dnaA框被证明对ColIb-P9的DNA复制并非必需。ColIb-P9的ssi信号是R1和R100质粒G位点的同源物。G位点的缺失导致拷贝数降低了1.5倍，这表明尽管该G位点并非必需，但对ColIb-P9 DNA的高效复制很重要。此外，ColIb-P9复制子与P307（RepFIC）复制子高度且广泛同源，并且在G位点区域周围与R100（RepFIIA）复制子高度同源。这些事实暗示了这些质粒进化而来的共同祖先。
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