Cymerman Iwona A, Meiss Gregor, Bujnicki Janusz M
Laboratory of Bioinformatics and Protein Engineering, International Institute of Molecular and Cell Biology, Trojdena 4, 02-109 Warsaw, Poland.
Bioinformatics. 2005 Nov 1;21(21):3959-62. doi: 10.1093/bioinformatics/bti659. Epub 2005 Sep 8.
DNase II is an endodeoxyribonuclease involved in apoptosis and essential for the mammalian development. Despite the understanding of biochemical properties of this enzyme, its structure and relationships to other protein families remain unknown.
Using protein fold-recognition we found that DNase II exhibits a catalytic domain common to the phospholipase D superfamily. Our model explains the available experimental data and provides the first structural platform for sequence-function analyses of this important nuclease.
脱氧核糖核酸酶II是一种参与细胞凋亡的内切脱氧核糖核酸酶，对哺乳动物的发育至关重要。尽管对该酶的生化特性已有了解，但其结构以及与其他蛋白质家族的关系仍不清楚。
利用蛋白质折叠识别技术，我们发现脱氧核糖核酸酶II具有磷脂酶D超家族共有的催化结构域。我们的模型解释了现有的实验数据，并为这一重要核酸酶的序列-功能分析提供了首个结构平台。
