Ciana Paolo, Brena Andrea, Sparaciari Paolo, Bonetti Elena, Di Lorenzo Diego, Maggi Adriana
Department of Pharmacological Sciences, Center of Excellence on Neurodegenerative Diseases, University of Milan, Italy.
Endocrinology. 2005 Dec;146(12):5144-50. doi: 10.1210/en.2005-0660. Epub 2005 Sep 8.
Diets lacking soy and alpha-alpha derivatives that are considered to be estrogen-free by standard bioassays (uterotrophic assay and vaginal opening) have been revealed to contain considerable amounts of compounds able to transcriptionally activate the estrogen receptors (ERs) and stimulate luciferase expression in several organs of the ERE-Luc reporter mouse. By molecular imaging, we show that ER activation is present in nonreproductive organs to an extent similar to that observed with the administration of 17beta-estradiol, and it is not influenced by orchiectomy or treatment with an aromatase inhibitor. This, together with the use of a completely synthetic diet, proves that the activation of ERs observed is due to estrogenic compounds present in commercial diets and that it is not a secondary event determined by food consumption and metabolism. The pervasiveness of estrogenic compounds in nature poses the question of how relevant and necessary is the daily ingestion of natural compounds active through the ERs for the maintenance of a correct metabolism in both male and female mammals.
通过标准生物测定法（子宫增重试验和阴道开口试验）被认为不含雌激素的缺乏大豆和苜蓿衍生物的饮食，已被发现含有大量能够转录激活雌激素受体（ERs）并在ERE-Luc报告基因小鼠的多个器官中刺激荧光素酶表达的化合物。通过分子成像，我们表明ER激活在非生殖器官中的程度与给予17β-雌二醇时观察到的程度相似，并且不受去势或芳香化酶抑制剂治疗的影响。这一点，连同使用完全合成的饮食，证明观察到的ER激活是由于商业饮食中存在的雌激素化合物，而不是由食物消耗和代谢决定的继发事件。自然界中雌激素化合物的普遍存在提出了一个问题，即对于维持雄性和雌性哺乳动物的正确代谢而言，每日摄入通过ERs起作用的天然化合物有多相关和必要。
