Strazisar Monika, Fir Maja, Golc-Wondra Alenka, Milivojevic Luka, Prosek Mirko, Abram Veronika
National Institute of Chemistry, Hajdrihova 19, Ljubljana, Slovenia.
J AOAC Int. 2005 Jul-Aug;88(4):1020-7.
The dietary sources of CoQ10 and the evaluation of CoQ10 in dairy products were characterized. For quantitation of CoQ10 in food samples, 2 liquid chromatography (LC) methods with UV and mass spectrometry (MS) detections were developed. LC with UV detection was performed at 25 degrees C on a Hyperclone ODS 5 microm 150 x 4.6 mm column with mobile phase consisting of methanol-ethanol-2-propanol (70 + 15 + 15, v/v/v). Flow rate was 1.0 mL/min. Retention time of CoQ10 was 10.9 +/- 0.1 min. The method was sensitive [limit of detection (LOD) = 0.2 mg/kg], reproducible [relative standard deviation (RSD) = 3:0%), and linear up to 25 mg/kg (R > 0.999). LC/MS analysis was performed on a LUNA C18 3 microm, 150 x 4.6 mm column, using mobile phase consisting of ethanol-dioxane-acetic acid (9 + 1 + 0.01, v/v/v), flow rate was 0.6 mL/min, and the retention time of CoQ10 was 4.1 +/- 0.1 min. Identification and quantitation were performed with a Finnigan-LCQ mass detector in positive atmospheric pressure chemical ionization mode. Mass spectra were obtained in selected-ion monitoring mode; molecular mass (M+H)+ m/z 863.4 +/- 1 was used for quantitative determination. MS detection is more sensitive than UV detection (LOD = 0.1 mg/kg), less reproducible (RSD = 4.0%), and linear in selected range. Analytical recoveries are 75-90% and depend on the ratio between the amount of fat in the matrix and the concentration of CoQ10 in the sample. Some soybean milk products were analyzed together with different cow, goat, and sheep milk products. Concentrations obtained with LC and LC/MS were compared with a few accessible results available from the literature. Concentrations varied from 0 ppm in soybean milk to nearly 2 ppm in fresh milk from local farms.
对辅酶Q10的膳食来源以及乳制品中辅酶Q10的评估进行了表征。为了对食品样品中的辅酶Q10进行定量分析，开发了2种采用紫外检测和质谱（MS）检测的液相色谱（LC）方法。采用紫外检测的液相色谱分析在25℃下于一根Hyperclone ODS 5μm 150×4.6mm色谱柱上进行，流动相由甲醇 - 乙醇 - 2 - 丙醇（70 + 15 + 15，v/v/v）组成。流速为1.0 mL/min。辅酶Q10的保留时间为10.9±0.1分钟。该方法灵敏[检测限（LOD）= 0.2 mg/kg]、可重现[相对标准偏差（RSD）= 3.0%]，并且在高达25 mg/kg的范围内呈线性（R > 0.999）。液相色谱/质谱分析在一根LUNA C18 3μm、150×4.6mm色谱柱上进行，使用由乙醇 - 二氧六环 - 乙酸（9 + 1 + 0.01，v/v/v）组成的流动相，流速为0.6 mL/min，辅酶Q10的保留时间为4.1±0.1分钟。使用Finnigan - LCQ质量检测器在正大气压化学电离模式下进行鉴定和定量分析。在选择离子监测模式下获得质谱图；分子量（M + H）+ m/z 863.4±1用于定量测定。质谱检测比紫外检测更灵敏（LOD = 0.1 mg/kg），重现性较差（RSD = 4.0%），并且在选定范围内呈线性。分析回收率为75 - 90%，并且取决于基质中脂肪含量与样品中辅酶Q10浓度之间的比例。将一些豆浆产品与不同的牛奶、羊奶和绵羊奶产品一起进行了分析。将液相色谱和液相色谱/质谱分析获得的浓度与文献中可得的一些可获取结果进行了比较。浓度范围从豆浆中的0 ppm到当地农场新鲜牛奶中的近2 ppm不等。
Zotero 插件