Sudha T, Teja V D, Gopal M, Rajesh M, Lakshmi V
Department of Microbiology, Nizam's Institute of Medical Sciences, Hyderabad, AP, India.
Clin Microbiol Infect. 2005 Oct;11(10):850-2. doi: 10.1111/j.1469-0691.2005.01231.x.
This study evaluated the TRI-DOT Rapid HIV test for the early detection of human immunodeficiency virus (HIV) infection in comparison with a fourth-generation ELISA (Vironostika HIV Uniform II). Of 23,609 sera, seven (0.03%) gave discordant results. Six of these were reactive only by the fourth-generation assay and were p24 antigen-positive by VIDAS DUO, Western blot and qualitative RT-PCR tests. The remaining discordant serum was considered to be false-positive by the TRI-DOT assay, as it was negative by repeat ELISA and Western blot tests. The sensitivity and specificity of the TRI-DOT test were 99.48% and 99.99%, respectively, compared with the fourth-generation ELISA.
本研究评估了TRI-DOT快速艾滋病毒检测法用于早期检测人类免疫缺陷病毒（HIV）感染的效果，并与第四代酶联免疫吸附测定法（Vironostika HIV Uniform II）进行了比较。在23609份血清样本中，有7份（0.03%）检测结果不一致。其中6份仅在第四代检测中呈阳性反应，且通过VIDAS DUO、免疫印迹法和定性逆转录-聚合酶链反应检测显示p24抗原呈阳性。其余一份结果不一致的血清样本被认为是TRI-DOT检测法出现的假阳性，因为重复酶联免疫吸附测定法和免疫印迹法检测结果均为阴性。与第四代酶联免疫吸附测定法相比，TRI-DOT检测法的灵敏度和特异性分别为99.48%和99.99%。
