Kurath Gael, Garver Kyle A, Corbeil Serge, Elliott Diane G, Anderson Eric D, LaPatra Scott E
U.S.G.S. Western Fisheries Research Center, 6505 NE 65th St., Seattle, WA 98115, USA.
Vaccine. 2006 Jan 16;24(3):345-54. doi: 10.1016/j.vaccine.2005.07.068. Epub 2005 Aug 9.
The DNA vaccine pIHNw-G encodes the glycoprotein of the fish rhabdovirus infectious hematopoietic necrosis virus (IHNV). Vaccine performance in rainbow trout was measured 3, 6, 13, 24, and 25 months after vaccination. At three months all fish vaccinated with 0.1 microg pIHNw-G had detectable neutralizing antibody (NAb) and they were completely protected from lethal IHNV challenge with a relative percent survival (RPS) of 100% compared to control fish. Viral challenges at 6, 13, 24, and 25 months post-vaccination showed protection with RPS values of 47-69%, while NAb seroprevalence declined to undetectable levels. Passive transfer experiments with sera from fish after two years post-vaccination were inconsistent but significant protection was observed in some cases. The long-term duration of protection observed here defined a third temporal phase in the immune response to IHNV DNA vaccination, characterized by reduced but significant levels of protection, and decline or absence of detectable NAb titers. Examination of multiple tissues showed an absence of detectable long-term histopathological damage due to DNA vaccination.
DNA疫苗pIHNw-G编码鱼类弹状病毒传染性造血器官坏死病毒（IHNV）的糖蛋白。在接种疫苗后3、6、13、24和25个月测量虹鳟鱼的疫苗性能。在三个月时，所有接种0.1微克pIHNw-G的鱼都能检测到中和抗体（NAb），并且它们完全受到保护，免受致死性IHNV攻击，与对照鱼相比，相对存活率（RPS）为100%。接种疫苗后6、13、24和25个月的病毒攻击显示出RPS值为47-69%的保护作用，而NAb血清阳性率下降到无法检测的水平。接种疫苗两年后用鱼血清进行的被动转移实验结果不一致，但在某些情况下观察到了显著的保护作用。此处观察到的长期保护期定义了对IHNV DNA疫苗免疫反应的第三个时间阶段，其特征是保护水平降低但仍显著，以及可检测到的NAb滴度下降或消失。对多个组织的检查表明，DNA疫苗接种未造成可检测到的长期组织病理学损伤。
