痣样基底细胞癌综合征（NBCCS）细胞经X射线照射后，SMT3A基因表达下调与DNA合成诱导相关。

Down-regulation of SMT3A gene expression in association with DNA synthesis induction after X-ray irradiation in nevoid basal cell carcinoma syndrome (NBCCS) cells.

作者信息

Sugaya Shigeru, Nakanishi Hiroshi, Tanzawa Hideki, Sugita Katsuo, Kita Kazuko, Suzuki Nobuo

机构信息

Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chiba 260-8670, Japan.

出版信息

Mutat Res. 2005 Oct 15;578(1-2):327-32. doi: 10.1016/j.mrfmmm.2005.06.027.

DOI:10.1016/j.mrfmmm.2005.06.027

PMID:16154602

Abstract

Fibroblast cells derived from nevoid basal carcinoma syndrome (NBCCS) patients show increased levels of DNA synthesis after X-ray irradiation. Genes, whose expression is modulated in association with the DNA synthesis induction, were searched by using PCR-based mRNA differential display analysis in one of the NBCCS cell lines, NBCCS1 cells. Decreased levels of SMT3A gene expression were found in X-ray-irradiated NBCCS1 cells. This decrease was also shown by RT-PCR analysis in another cell line, NBCCS3 cells. In addition to NBCCS cells, normal fibroblast cells showed the DNA synthesis induction after X-ray irradiation when they were treated with antisense oligonucleotides (AO) for SMT3A. However, treatment of normal fibroblasts with the random oligonucleotides (RO) resulted in decreased levels of DNA synthesis after X-ray irradiation. Thus, down-regulation of SMT3A gene expression may be involved in the DNA synthesis induction after X-ray irradiation in the NBCCS cells at least tested.

摘要

源自痣样基底细胞癌综合征（NBCCS）患者的成纤维细胞在X射线照射后显示出DNA合成水平增加。在其中一个NBCCS细胞系NBCCS1细胞中，通过基于PCR的mRNA差异显示分析，搜索了其表达与DNA合成诱导相关而被调节的基因。在X射线照射的NBCCS1细胞中发现SMT3A基因表达水平降低。在另一个细胞系NBCCS3细胞中，RT-PCR分析也显示了这种降低。除了NBCCS细胞外，正常成纤维细胞在用针对SMT3A的反义寡核苷酸（AO）处理后，在X射线照射后也显示出DNA合成诱导。然而，用随机寡核苷酸（RO）处理正常成纤维细胞导致X射线照射后DNA合成水平降低。因此，至少在所测试的NBCCS细胞中，SMT3A基因表达的下调可能参与了X射线照射后的DNA合成诱导。