Chen Mien-Cheng, Chang Hsueh-Wen, Wu Chiung-Jen, Yang Cheng-Hsu, Yu Teng-Hung, Chen Chien-Jen, Hung Wei-Chin
Division of Cardiology, Department of Internal Medicine, Chang Gung Memorial Hospital, Niao Sung Hsiang, Kaohsiung, Taiwan, ROC.
Cardiology. 2005;104(4):171-5. doi: 10.1159/000088104. Epub 2005 Sep 7.
The study population consisted of 16 patients with rheumatic mitral stenosis undergoing percutaneous transluminal mitral valvuloplasty (group 1). The plasma levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10) in the femoral vein and the right and left atria before valvuloplasty were determined by ELISA. Additionally, we measured plasma concentrations of TNF-alpha and IL-10 in the venous blood obtained from 19 control patients, including 12 healthy volunteers in sinus rhythm (group 2) and 7 patients in permanent lone atrial fibrillation (group 3). The venous plasma levels of TNF-alpha were significantly elevated in group 1 patients compared with group 2 patients (p < 0.002). Correlation analysis demonstrated that there was a significantly direct relationship between the plasma TNF-alpha and IL-10 concentrations in the left atrial, right atrial and peripheral venous blood (p < 0.008, r = 0.640; p < 0.04, r = 0.538; p< 0.03, r = 0.571, respectively). In conclusion, the plasma concentrations of TNF-alpha of patients with rheumatic mitral stenosis were significantly higher than those of healthy volunteers. In addition, there was a significantly direct relationship between the soluble TNF-alpha and IL-10 concentrations in the atrial and peripheral venous blood, indicating a balance between circulating TNF-alpha and IL-10 levels in patients with rheumatic mitral stenosis.
研究人群包括16例接受经皮腔内二尖瓣成形术的风湿性二尖瓣狭窄患者（第1组）。通过酶联免疫吸附测定法（ELISA）测定瓣膜成形术前股静脉以及左右心房中肿瘤坏死因子-α（TNF-α）和白细胞介素-10（IL-10）的血浆水平。此外，我们测量了从19例对照患者获取的静脉血中TNF-α和IL-10的血浆浓度，其中包括12例窦性心律的健康志愿者（第2组）和7例永久性孤立性心房颤动患者（第3组）。与第2组患者相比，第1组患者的静脉血浆TNF-α水平显著升高（p < 0.002）。相关性分析表明，左心房、右心房和外周静脉血中的血浆TNF-α与IL-10浓度之间存在显著的直接关系（分别为p < 0.008，r = 0.640；p < 0.04，r = 0.538；p < 0.03，r = 0.571）。总之，风湿性二尖瓣狭窄患者的血浆TNF-α浓度显著高于健康志愿者。此外，心房和外周静脉血中可溶性TNF-α与IL-10浓度之间存在显著的直接关系，表明风湿性二尖瓣狭窄患者循环中的TNF-α和IL-10水平之间存在平衡。
