甲硫氨酰 - tRNA合成酶识别反密码子的结构基础。

Structural basis for anticodon recognition by methionyl-tRNA synthetase.

作者信息

Nakanishi Kotaro, Ogiso Yuri, Nakama Takashi, Fukai Shuya, Nureki Osamu

机构信息

Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama-shi, Kanagawa 226-8501, Japan.

出版信息

Nat Struct Mol Biol. 2005 Oct;12(10):931-2. doi: 10.1038/nsmb988. Epub 2005 Sep 11.

DOI:10.1038/nsmb988

PMID:16155581

Abstract

In the 2.7-A resolution crystal structure of methionyl-tRNA synthetase (MetRS) in complex with tRNA(Met) and a methionyl-adenylate analog, the tRNA anticodon loop is distorted to form a triple-base stack comprising C34, A35 and A38. A tryptophan residue stacks on C34 to extend the triple-base stack. In addition, C34 forms Watson-Crick-type hydrogen bonds with Arg357. This structure resolves the longstanding question of how MetRS specifically recognizes tRNA(Met).

摘要

在甲硫氨酰 - tRNA合成酶（MetRS）与tRNA(Met)及甲硫氨酰 - 腺苷酸类似物复合物的2.7埃分辨率晶体结构中，tRNA反密码子环发生扭曲，形成了一个由C34、A35和A38组成的三碱基堆积。一个色氨酸残基堆积在C34上以扩展三碱基堆积。此外，C34与Arg357形成沃森 - 克里克型氢键。该结构解决了长期以来关于MetRS如何特异性识别tRNA(Met)的问题。