雷公藤甲素通过降低尿激酶型纤溶酶原激活剂表达对血管生成的影响

[Effects of triptolide on anti-angiogenesis by reducing expression of urokinase plasminogen activator].

作者信息

Ding Yi, Zhang Jiancheng, Hou Lijun, Zhang Jie, Wang Qiulin, Wang Shuren

机构信息

Department of Pathophysiology, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu, China.

出版信息

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2005 Aug;22(4):778-81.

PMID:16156271

Abstract

In order to unveil the anti-angiogenic mechanism of triptolide, we investigated the effects of triptolide on the proliferation, migration, angiogenesis and u-PA expression of cultured HUVECs. MTT assay found that triptolide could inhibit the proliferation of HUVECs, and three-dimensional culture system revealed that triptolide could curb the migration of HUVECs. The chick embryo chorioallantoic membrane (CAM) test showed that triptolide could inhibit angiogenesis. Real time quantitive RT-PCR showed the expression of u-PA of HUVECs was down-regulated by triptolide. Therefore, triptolide may inhibit the proliferation and migration of endothelial cell by way of reducing the expression of u-PA. These findings may conduce to the elucidation of the anti-angiogenic mechanism of triptolide.

摘要

为揭示雷公藤甲素的抗血管生成机制，我们研究了雷公藤甲素对培养的人脐静脉内皮细胞（HUVECs）增殖、迁移、血管生成及尿激酶型纤溶酶原激活剂（u-PA）表达的影响。MTT 法检测发现雷公藤甲素可抑制 HUVECs 的增殖，三维培养系统显示雷公藤甲素可抑制 HUVECs 的迁移。鸡胚绒毛尿囊膜（CAM）试验表明雷公藤甲素可抑制血管生成。实时定量 RT-PCR 显示雷公藤甲素可下调 HUVECs 中 u-PA 的表达。因此，雷公藤甲素可能通过降低 u-PA 的表达来抑制内皮细胞的增殖和迁移。这些发现可能有助于阐明雷公藤甲素的抗血管生成机制。