Prickett M D, Prestwood A K, Hoenig M
Department of Parasitology, College of Veterinary Medicine, University of Georgia, Athens 30602.
Vet Parasitol. 1992 Apr;42(1-2):41-51. doi: 10.1016/0304-4017(92)90101-e.
Sixteen 2-month-old pigs were divided into four equal groups and infected with either 500,000, 1,000,000 or 3,000,000 sporocysts of Sarcocystis miescheriana. Four pigs served as uninfected controls. Pigs were bled weekly and serum was collected beginning 14 days prior to infection and continuing until 63 days after infection. Body fat composition, as measured by the specific gravity of the carcass, was not affected by infection. There were no significant effects of infection on serum concentrations of glucose, insulin, triglycerides, and total, high-density lipoprotein (HDL) and low-density lipoprotein (LDL) cholesterol. A slight depression in HDL cholesterol occurred during the acute phase of infection. Tumor necrosis factor (TNF) was not detected in serum from infected swine when assayed by a cytotoxicity assay using TNF-sensitive WEHI 164 clone 13 cells. Attempts to stimulate TNF production in RAW 264.7 cells with parasitic lysates gave mixed results. This study suggests that the disruption of lipid metabolism is not the primary cause of growth retardation in growing swine infected with S. miescheriana.
将16头2月龄仔猪分为四组，每组数量相等，分别感染500,000、1,000,000或3,000,000个米氏肉孢子虫孢子囊。4头猪作为未感染对照。每周对猪进行采血，从感染前14天开始收集血清，持续至感染后63天。通过胴体比重测量的体脂组成不受感染影响。感染对血清葡萄糖、胰岛素、甘油三酯以及总胆固醇、高密度脂蛋白（HDL）和低密度脂蛋白（LDL）胆固醇浓度均无显著影响。感染急性期HDL胆固醇略有下降。当使用对TNF敏感的WEHI 164克隆13细胞通过细胞毒性试验检测时，在感染猪的血清中未检测到肿瘤坏死因子（TNF）。用寄生虫裂解物刺激RAW 264.7细胞产生TNF的尝试结果不一。本研究表明，脂质代谢紊乱不是感染米氏肉孢子虫的生长猪生长迟缓的主要原因。
