Han Kyoung-Sik, Kim Younghoon, Choi Seungkook, Oh Sejong, Park Sungsu, Kim Sae-Hun, Whang Kwang-Youn
Division of Food Science, Korea University, 136-701, Seoul, Korea.
Biotechnol Lett. 2005 Aug;27(16):1183-8. doi: 10.1007/s10529-005-0014-z.
A rapid molecular approach was developed for the initial identification of Lactobacillus acidophilus strains which are difficult to identify using a single biochemical test. The 16S-23S rRNA intergenic spacer regions and flanking 23S rRNA genes of 19 strains of lactobacilli were amplified and the nucleotide sequences and restriction site polymorphisms were analyzed. AluI was the most useful of the restriction enzymes analyzed and produced reproducible digestion profiles in the L. helveticus, L. plantarum, and L. casei groups, as well as in L. acidophilus. This restriction fragment length polymorphism method may be useful for the identification of L. acidophilus strains in dairy products.
开发了一种快速分子方法，用于初步鉴定嗜酸乳杆菌菌株，这些菌株难以通过单一生化试验进行鉴定。对19株乳杆菌的16S-23S rRNA基因间隔区和侧翼23S rRNA基因进行了扩增，并分析了核苷酸序列和限制性酶切位点多态性。在分析的限制性酶中，AluI最为有用，它在瑞士乳杆菌、植物乳杆菌、干酪乳杆菌组以及嗜酸乳杆菌中产生了可重复的酶切图谱。这种限制性片段长度多态性方法可能有助于鉴定乳制品中的嗜酸乳杆菌菌株。
