Gao Yu-Long, Ju Xing-Rong, Jiang Han-Hu
College of Food Science and Engineering, Nanjing University of Finance and Economics, Railway North Street No. 128, Nanjing, 210003, Jiangsu Province, P.R. China.
Appl Microbiol Biotechnol. 2006 May;70(6):670-8. doi: 10.1007/s00253-005-0143-2. Epub 2005 Sep 13.
Previous unpublished experimental results of fractional factorial experiments showed that significant external factors affecting high-pressure processing (HPP) inactivation were pressure, temperature, and pressure holding time. Based on these results, response surface methodology (RSM) was employed in the present work, and a quadratic equation for HPP inactivation was built with RSM. By analyzing response surface plots and their corresponding contour plots and by solving the quadratic equation, experimental values were shown to be significantly in good agreement with predicted values, since the adjusted determination coefficient (RAdj2) was 0.9812 and the level of significance was P<0.0001. Optimum process parameters for a 6-log cycle reduction of Listeria monocytogenes were obtained: pressure, 448.0 MPa; temperature, 41 degrees C; and pressure holding time, 11 min. The adequacy of the model equation in predicting optimum response values was verified effectively by validation data.
先前未发表的部分因子实验的实验结果表明，影响高压处理（HPP）失活的显著外部因素是压力、温度和保压时间。基于这些结果，本研究采用了响应面法（RSM），并用RSM建立了HPP失活的二次方程。通过分析响应面图及其相应的等高线图，并求解二次方程，实验值与预测值显示出显著的良好一致性，因为调整后的决定系数（RAdj2）为0.9812，显著性水平为P<0.0001。获得了使单核细胞增生李斯特菌减少6个对数循环的最佳工艺参数：压力448.0MPa；温度41℃；保压时间11分钟。验证数据有效地验证了模型方程在预测最佳响应值方面的适用性。
