Wang Hai-Lei, Li Zong-Yi, Guo Wei-Yun, Wang Zhen-Yu, Pan Feng
College of Life Sciences, Henan Normal University, Xinxiang 453002, China.
J Environ Sci (China). 2005;17(4):620-2.
Since the ability to degrade lignin with one kind of white-rot fungi or bacteria was very limited, superior mixed flora's ability to degrade lignin was investigated by an orthogonal experiment in this paper. The results showed that superior mixed flora reinforced the ability to degrade lignin, the degradation rates of both sample 9 and 10 were beyond 80% on the day 9. The cooperation between lignin peroxidase (LiP), Mn-dependent peroxidase (MnP) and laccase (Lac) for lignin degradation was also studied. By examining the activities of three enzymes produced by superior mixed flora, it was found that Lac was a key enzyme in the process of biological degradation of lignin but Lip was not; the enzyme activity ratios of Lac/MnP and Lac/LiP were significantly correlative with the degradation rate of lignin at the 0.01 level; and the ratio of MnP/LiP was an important factor affecting the degradation rate of lignin.
由于单一的白腐真菌或细菌降解木质素的能力非常有限，本文通过正交试验研究了优势混合菌群降解木质素的能力。结果表明，优势混合菌群增强了木质素降解能力，样品9和10在第9天的降解率均超过80%。还研究了木质素过氧化物酶（LiP）、锰依赖过氧化物酶（MnP）和漆酶（Lac）在木质素降解中的协同作用。通过检测优势混合菌群产生的三种酶的活性，发现Lac是木质素生物降解过程中的关键酶，而LiP不是；Lac/MnP和Lac/LiP的酶活性比与木质素降解率在0.01水平上显著相关；MnP/LiP的比值是影响木质素降解率的重要因素。
