González Pereyra María Laura, Cariddi L N, Ybarra F, Isola M C, Demo M S, Sabini L, Maldonado A M
Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Provincia de Córdoba, Argentina.
Rev Alerg Mex. 2005 May-Jun;52(3):105-12.
Minthostachys verticillata (Griseb.) Epling is a South American traditional medicinal herb used as digestive, anti-spasmodic, anti-inflammatory and bronchial dilator agent among other uses. Its anti-microbial activity against staphylococcal strains and its anti-viral properties against HVS-1 and strain RC/79 of PrV have been demonstrated.
To determine the immunomodulating ability of M. verticillata decoction and essential oil. As a complementary study, the main constituents of the essential oil were identified.
Lymphocyte-proliferating activity of both vegetal derivatives was tested and compared with cellular expansion induced by PHA, Pokeweed, CGB in cytomorphological study. A non-stimulate culture was used as control reference. The score of lymphocyte clusters and colonies was performed using the method described by Lange. Among proliferated cells, LT CD8+ subpopulation was characterized by direct immunofluorescence. The in vitro degranulant ability of the vegetal fractions was tested on basophils from allergic and non-allergic individuals sensitized to environmental fungi. Essential oil components of M. verticillata were identified by gas chromatography technique.
M. verticillata derivatives reached higher proliferation levels compared to non-stimulated cultures, showed mitogenic activity and induced cluster and colony formation similar to PHA, Pokeweed and CGB. Cells that proliferated after stimulation with derivatives showed 40% of LT CD8+. Tested concentrations of decoction and essential oil did not reach minimum degranulation indexes over basophils, from both allergic and non-allergic individuals. Gas chromatography analysis revealed the presence of pulegone and menthone as the main constituents.
M. verticillata derivatives were mitogenic over LT, inducing significant cluster and colony formation. There was no evidence of degranulating ability over basophils at the concentrations tested. We assume that the derivatives from M. verticillata would induce Th1 deviation in cellular cultures from allergic patients, which would diminish hypersensitivity reactions. Some of the compounds of the essential oil revealed by gas chromatography analysis may be responsible of the biological activity of these products.
轮生薄荷（Minthostachys verticillata (Griseb.) Epling）是一种南美传统药草，具有助消化、抗痉挛、抗炎及支气管扩张等功效。其对葡萄球菌菌株的抗菌活性以及对疱疹病毒1型（HVS-1）和伪狂犬病病毒（PrV）RC/79株的抗病毒特性已得到证实。
确定轮生薄荷水煎剂和精油的免疫调节能力。作为一项补充研究，鉴定了精油的主要成分。
在细胞形态学研究中，测试了这两种植物提取物的淋巴细胞增殖活性，并与由植物血凝素（PHA）、商陆、CGB诱导的细胞扩增进行比较。以未刺激的培养物作为对照参考。使用Lange描述的方法对淋巴细胞簇和集落进行评分。在增殖细胞中，通过直接免疫荧光对LT CD8 +亚群进行表征。在对环境真菌致敏的过敏和非过敏个体的嗜碱性粒细胞上测试了植物提取物的体外脱颗粒能力。通过气相色谱技术鉴定了轮生薄荷的精油成分。
与未刺激的培养物相比，轮生薄荷提取物达到了更高的增殖水平，表现出促有丝分裂活性，并诱导了类似于PHA、商陆和CGB的簇和集落形成。用提取物刺激后增殖的细胞显示40%为LT CD8 +。测试浓度的水煎剂和精油对过敏和非过敏个体的嗜碱性粒细胞均未达到最低脱颗粒指数。气相色谱分析显示，主要成分是胡薄荷酮和薄荷酮。
轮生薄荷提取物对LT具有促有丝分裂作用，诱导了显著的簇和集落形成。在所测试的浓度下，没有证据表明其对嗜碱性粒细胞有脱颗粒能力。我们推测，轮生薄荷提取物会在过敏患者的细胞培养物中诱导Th1偏移，这将减少过敏反应。气相色谱分析揭示的精油中的一些化合物可能是这些产品生物活性的原因。
