Takegawa Yasuhiro, Deguchi Kisaburo, Nakagawa Hiroaki, Nishimura Shin-Ichiro
Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 001-0021, Japan.
Anal Chem. 2005 Sep 15;77(18):6062-8. doi: 10.1021/ac050843e.
A novel N-linked oligosaccharide (N-glycan) with "beta1-4 bisecting branch (galactose beta1-4 bisecting N-acetylglucosamine)" was found in human serum IgG. Its structure was efficiently analyzed by using beta-galactosidase digestion, a MSn spectral library database, and negative-ion MS2 spectral matching. For confirmation, the novel N-glycan was synthesized by using an expected standard N-glycan (acceptor), UDP-galactose (donor), and beta1-4 galactosyltransferase. This work also demonstrates that the MSn spectral library database, in particular, negative-ion MS2 spectral matching, can efficiently reduce the number of specific, sequential exoglycosidase digestions required and is useful for rapid structural analysis of unknown glycans not in the database.
在人血清IgG中发现了一种具有“β1-4分支（半乳糖β1-4连接N-乙酰葡糖胺）”的新型N-连接寡糖（N-聚糖）。通过使用β-半乳糖苷酶消化、MSn光谱库数据库和负离子MS2光谱匹配对其结构进行了有效分析。为了进行确认，使用预期的标准N-聚糖（受体）、UDP-半乳糖（供体）和β1-4半乳糖基转移酶合成了这种新型N-聚糖。这项工作还表明，MSn光谱库数据库，特别是负离子MS2光谱匹配，可以有效减少所需的特定顺序外切糖苷酶消化的数量，并且对于数据库中不存在的未知聚糖的快速结构分析很有用。
