布尼亚姆韦拉病毒信使核糖核酸转录信号存在于3'和5'末端区域，并允许从一个模型核糖核酸片段进行双义转录。
The Bunyamwera virus mRNA transcription signal resides within both the 3' and the 5' terminal regions and allows ambisense transcription from a model RNA segment.
作者信息
Barr John N, Rodgers John W, Wertz Gail W
机构信息
Department of Microbiology, University of Alabama School of Medicine, BBRB Room 360/Box 17, 845 19th Street South, Birmingham AL 35294-2170, USA.
出版信息
J Virol. 2005 Oct;79(19):12602-7. doi: 10.1128/JVI.79.19.12602-12607.2005.
Abstract
Bunyamwera virus (BUNV) is the prototype of the Bunyaviridae family of RNA viruses. BUNV genomic strands are templates for both replication and transcription, whereas the antigenomic RNAs serve only as templates for replication. By mutagenesis of model templates, we showed that the BUNV transcription promoter comprises elements within both the 3' and the 5' nontranslated regions. Using this information, we designed a model ambisense BUNV segment that transcribed BUNV S mRNA from the genomic strand and green fluorescent protein (GFP) mRNA from the antigenome. Demonstration of GFP expression showed that this ambisense strategy represents a viable approach for generating BUNV segments able to express additional proteins.
摘要
布尼亚姆韦拉病毒（BUNV）是RNA病毒布尼亚病毒科的原型。BUNV基因组链既是复制模板也是转录模板，而反基因组RNA仅作为复制模板。通过对模型模板进行诱变，我们发现BUNV转录启动子包含3'和5'非翻译区内的元件。利用这一信息，我们设计了一个模型双义BUNV片段，该片段从基因组链转录BUNV S mRNA，从反基因组转录绿色荧光蛋白（GFP）mRNA。GFP表达的证明表明，这种双义策略是产生能够表达额外蛋白质的BUNV片段的可行方法。
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本文引用的文献
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Role of the conserved nucleotide mismatch within 3'- and 5'-terminal regions of Bunyamwera virus in signaling transcription.布尼亚姆韦拉病毒3'和5'末端区域内保守核苷酸错配在信号转录中的作用。
J Virol. 2005 Mar;79(6):3586-94. doi: 10.1128/JVI.79.6.3586-3594.2005.
2
Bunyamwera bunyavirus RNA synthesis requires cooperation of 3'- and 5'-terminal sequences.布尼亚姆韦拉布尼亚病毒的RNA合成需要3'端和5'端序列的协同作用。
J Virol. 2004 Feb;78(3):1129-38. doi: 10.1128/jvi.78.3.1129-1138.2004.
3
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