Kanai S, Okamoto H, Tamaura Y, Yamazaki S, Inada Y
Thromb Haemost. 1979 Dec 21;42(4):1153-8.
Fibrin polymers formed from fibrinogen with thrombin in the presence of EDTA were suspended in a medium containing glucose, arabic gum and imidazole-HCl buffer and were sonicated at 20 kHz for 20 min to make a suspension containing fibrin particles of small size. The fibrin suspension was used as a substrate of plasmin for determining the enzymic activity of plasmin and plasminogen activated with urokinase. The kinetic study on the reaction of the fibrin particles with plasmin in the presence and the absence of fibrinogen revealed that Km value of fibrin for plasmin is 4.2 x 10(-7) M and the Ki value of fibrinogen is 1.2 x 10(-5) M.
在乙二胺四乙酸（EDTA）存在的情况下，由纤维蛋白原与凝血酶形成的纤维蛋白聚合物悬浮于含有葡萄糖、阿拉伯胶和咪唑 - 盐酸缓冲液的介质中，并在20千赫下超声处理20分钟，以制成含有小尺寸纤维蛋白颗粒的悬浮液。该纤维蛋白悬浮液用作纤溶酶的底物，用于测定纤溶酶和经尿激酶激活的纤溶酶原的酶活性。对纤维蛋白颗粒在存在和不存在纤维蛋白原的情况下与纤溶酶反应的动力学研究表明，纤溶酶作用于纤维蛋白的米氏常数（Km值）为4.2×10⁻⁷ M，纤维蛋白原的抑制常数（Ki值）为1.2×10⁻⁵ M。
