Wojtkowska Malgorzata, Szczech Natalia, Stobienia Olgierd, Jarmuszkiewicz Wieslawa, Budzinska Małgorzata, Kmita Hanna
Laboratory of Bioenergetics, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Fredry 10, 61-701, Poznan, Poland.
J Bioenerg Biomembr. 2005 Aug;37(4):261-8. doi: 10.1007/s10863-005-6636-y.
It is suggested that in the course of the TOM complex evolution at least two lineages have appeared: the animal-fungal and green plant ones. The latter involves also the TOM complexes of algae and protozoans. The amoeba Acanthamoeba castellanii is a free-living non-photosynthetic soil protozoan, whose mitochondria share many bioenergetic properties with mitochondria of plants, animals and fungi. Here, we report that a protein complex, identified electrophysiologically as the A. castellanii TOM complex, contains a homologue of yeast/animal Tom 70. Further, molecular weight of the complex (about 500 kDa) also points to A. castellanii evolutionary relation with fungi and animal. Thus, the data indicates that the TOM complex of A. castellanii is not a typical example of the protozoan TOM complex.
有人认为，在TOM复合体的进化过程中至少出现了两个谱系：动物-真菌谱系和绿色植物谱系。后者还包括藻类和原生动物的TOM复合体。阿米巴虫卡氏棘阿米巴是一种自由生活的非光合土壤原生动物，其线粒体与植物、动物和真菌的线粒体具有许多生物能量特性。在此，我们报告，通过电生理学鉴定为卡氏棘阿米巴TOM复合体的一种蛋白质复合体含有酵母/动物Tom 70的同源物。此外，该复合体的分子量（约500 kDa）也表明卡氏棘阿米巴与真菌和动物存在进化关系。因此，数据表明卡氏棘阿米巴的TOM复合体不是原生动物TOM复合体的典型例子。
