Al-Dirbashi Osama Y, Aboul-Enein Hassan Y, Al-Odaib Ahmed, Jacob Minnie, Rashed Mohamed S
Department of Genetics, King Faisal Specialist Hospital and Research Centre, PO Box 3354, Riyadh 11211, Saudi Arabia.
Biomed Chromatogr. 2006 Apr;20(4):365-8. doi: 10.1002/bmc.571.
A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of ziprasidone (ZIP) in human plasma was developed. ZIP and N-methyl ziprasidone as internal standard (IS) were extracted from alkalinized plasma using tert- butyl methyl ether. Separation was performed isocratically on a C8 column with 90% acetonitrile containing 2 mmol/L ammonium acetate as a mobile phase with a total run time of 2.5 min. MS/MS transitions of m/z 413 --> 194 and m/z 427 --> 177 of the analyte and internal standard were used for quantification. Confirmatory ions of m/z 413 --> 177 and m/z 427 --> 180 were collected as well. The calibration curve based on peak-area ratio was linear up to at least 200 ng/mL with a detection limit of 0.1 ng/mL. The method showed satisfactory reproducibility with a coefficient of variation of less than 5%. The method was successfully applied to the analysis of ZIP in spiked human plasma.
建立了一种液相色谱-串联质谱（LC-MS/MS）法测定人血浆中的齐拉西酮（ZIP）。采用叔丁基甲醚从碱化血浆中提取ZIP和作为内标（IS）的N-甲基齐拉西酮。在C8柱上以含2 mmol/L醋酸铵的90%乙腈为流动相进行等度洗脱，总运行时间为2.5分钟。分析物和内标的m/z 413→194和m/z 427→177的MS/MS转换用于定量。还收集了m/z 413→177和m/z 427→180的确证离子。基于峰面积比的校准曲线在至少200 ng/mL范围内呈线性，检测限为0.1 ng/mL。该方法具有令人满意的重现性，变异系数小于5%。该方法成功应用于加标人血浆中ZIP的分析。
