Kollers S, Mégy K, Rocha D
Sygen International plc, Sygen Cambridge Research Laboratory, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK.
Anim Genet. 2005 Oct;36(5):426-31. doi: 10.1111/j.1365-2052.2005.01329.x.
More than 5500 pig single nucleotide polymorphisms (SNPs) were recently identified and deposited in the public domain. To test the usefulness of these public SNPs, 109 SNPs were analysed for polymorphism within six commercial pig populations. A functional polymerase chain reaction (PCR) assay was obtained for 103 SNPs and it was possible to validate c. 59% by PCR-restriction fragment length polymorphism. Furthermore, polymorphism was found using a relatively limited number of genomic DNA samples, indicating that these polymorphisms are segregating at a useful frequency in these populations. The high percentage of validated markers demonstrates the utility of these public pig SNPs to identify loci responsible for economically important traits in commercial pig populations.
最近鉴定出5500多个猪单核苷酸多态性（SNP）并公开存放。为了测试这些公开SNP的实用性，对六个商业猪群体中的109个SNP进行了多态性分析。针对103个SNP获得了功能性聚合酶链反应（PCR）检测方法，并且通过PCR-限制性片段长度多态性有可能验证约59%。此外，使用相对有限数量的基因组DNA样本发现了多态性，这表明这些多态性在这些群体中以有用的频率分离。高比例的已验证标记证明了这些公开的猪SNP在鉴定商业猪群体中经济重要性状相关基因座方面的实用性。
